Detection of rubella antibodies in human serum by the indirect fluorescent antibody technique [PDF]
Archiv f�r die gesamte Virusforschung, 1965 Antibodies for rubella virus were detected in human serum and titrated by the indirect method of immunofluorescence using a chronically infected, continuous line of monkey kidney cells as antigen. Positive reactions were obtained with convalescent or post-exposure specimens while acute sera and those from unexposed individuals were negative.
Hunein F. Maassab +3 more
openaire +4 more sources
Fluorescent humanized anti-CEA antibody specifically labels metastatic pancreatic cancer in a patient-derived orthotopic xenograft (PDOX) mouse model. [PDF]
, 2018 Pancreatic cancer is a highly lethal disease in part due to incomplete tumor resection. Targeting by tumor-specific antibodies conjugated with a fluorescent label can result in selective labeling of cancer in vivo for surgical navigation.
Amirfakhri, Siamak +12 more
core
Vitronectin at sites of cell-substrate contact in cultures of rat myotubes [PDF]
, 1986 Affinity-purified antibodies to the serum glycoprotein, vitronectin, were used to study sites of cell-substrate contact in cultures of rat myotubes and fibroblasts.
Baetscher, Manfred +2 more
core +1 more source
Sequence-based deep learning antibody design for in silico antibody affinity maturation [PDF]
arXiv, 2021 Antibody therapeutics has been extensively studied in drug discovery and development within the past decades. One increasingly popular focus in the antibody discovery pipeline is the optimization step for therapeutic leads. Both traditional methods and in silico approaches aim to generate candidates with high binding affinity against specific target ...
arxiv
Signal stability of Cy3 and Cy5 on antibody microarrays
Proteome Science, 2006 Background The antibody microarray technique is a newly emerging proteomics tool for differential protein expression analyses that uses fluorescent dyes Cy 3 and Cy 5.
Kim Caroline +2 more
doaj +1 more source
Evaluating Zero-Shot Scoring for In Vitro Antibody Binding Prediction with Experimental Validation [PDF]
arXiv, 2023 The success of therapeutic antibodies relies on their ability to selectively bind antigens. AI-based antibody design protocols have shown promise in generating epitope-specific designs. Many of these protocols use an inverse folding step to generate diverse sequences given a backbone structure.
arxiv
Single-cell western blotting. [PDF]
, 2014 To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E +5 more
core +2 more sources
Potential Neutralizing Antibodies Discovered for Novel Corona Virus Using Machine Learning [PDF]
arXiv, 2020 The fast and untraceable virus mutations take lives of thousands of people before the immune system can produce the inhibitory antibody. Recent outbreak of novel coronavirus infected and killed thousands of people in the world. Rapid methods in finding peptides or antibody sequences that can inhibit the viral epitopes of COVID-19 will save the life of ...
arxiv
Best practices for machine learning in antibody discovery and development [PDF]
arXiv, 2023 Over the past 40 years, the discovery and development of therapeutic antibodies to treat disease has become common practice. However, as therapeutic antibody constructs are becoming more sophisticated (e.g., multi-specifics), conventional approaches to optimisation are increasingly inefficient.
arxiv
Visualizing septins in early Drosophila embryos [PDF]
Methods in Cell Biology, Elsevier, 2016, Septins, 136, pp.183, 2016 Functional studies in Drosophila have been key for establishing a role for the septin family of proteins in animal cell division and thus extending for the first time observations from the budding yeast to animal cells. Visualizing the distribution of specific septins in different Drosophila tissues and, in particular, in the Drosophila embryo ...
arxiv +1 more source