Results 61 to 70 of about 422,292 (362)

Signal stability of Cy3 and Cy5 on antibody microarrays

Proteome Science, 2006
Background The antibody microarray technique is a newly emerging proteomics tool for differential protein expression analyses that uses fluorescent dyes Cy 3 and Cy 5.
Kim Caroline, Sivanandam Thamil Mani, Gu Qiang   +2 more
doaj   +1 more source

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring. [PDF]

, 2019
The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously ...
Chen, Ching, Chen, Irwin, Chong, Su, Hamburger, Agi, Jess, Philip, Jorgolli, Marsela, Le, Kim, Lin, Fen-Fen, Miranda, Les P, Mock, Marissa, Munro, Trent, Nevill, Tanner, Stevens, Jennitte, Tagari, Philip, Tan, Christopher, Winters, Aaron, Wu, Ming, Xu, Han   +17 more
core   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Patterns of gene expression in schistosomes: localization by whole mount in situ hybridization [PDF]

, 2007
rom the identification of genes to the characterization of their functions and interactions. Developmental biologists have long used whole mount in situ hybridization (WISH) to determine gene expression patterns, as a vital tool for formulating and ...
Davis, G. P. DILLON, H. V. ISAACS, J. C. ILLES, Pownall, R. A. WILSON, Skelly, Wilson   +7 more
core   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Detection of cryptosporidium oocysts in water and environmental concentrates [PDF]

, 1997
Whilst current methods for the isolation and enumeration of Cryptosporidium spp. oocysts in water have provided some insight into their occurrence and significance, they are regarded as being inefficient, variable and time-consuming, with much of the ...
Smith, H.V.
core  

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F., Hunka, Julia, Riley, John T   +2 more
core   +1 more source

Differentiation of ruminant transmissible spongiform encephalopathy isolate types, including bovine spongiform encephalopathy and CH1641 scrapie [PDF]

, 2010
With increased awareness of the diversity of transmissible spongiform encephalopathy (TSE) strains in the ruminant population, comes an appreciation of the need for improved methods of differential diagnosis. Exposure to bovine spongiform encephalopathy (
Bossers, A., Jacobs, J.G., Keulen, L.J.M., van, Langeveld, J.P.M., Sauer, M., Tang, Y.   +5 more
core   +2 more sources

Mitochondrial fatty acid oxidation is stimulated by red light irradiation

FEBS Letters, EarlyView.
Light at different wavelengths has distinct effects on keratinocyte viability and metabolism. UVA light abrogates metabolic fluxes. Blue and green light have no effect on metabolic fluxes, while red light enhanced oxidative phosphorylation by promoting fatty acid oxidation. Keratinocytes are the primary constituents of sunlight‐exposed epidermis.
Manuel Alejandro Herrera, Camille C. Caldeira da Silva, Maiza Von Denz, Mauricio S. Baptista, Alicia J. Kowaltowski   +4 more
wiley   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source