Campylobacteriosis in beef cattle in Alentejo – preliminary assessment [PDF]
The aim of this study was to assess the prevalence of infection by Campylobacter fetus in beef cattle reared in extensive systems in Alentejo region by direct fluorescent antibody test (DFAT), a technique widely used for determining the prevalence of ...
Pinto Coelho, M+2 more
core
Imaging the developing human external and internal urogenital organs with light sheet fluorescence microscopy. [PDF]
Technological advances in three-dimensional (3D) reconstruction techniques have previously enabled paradigm shifts in our understanding of human embryonic and fetal development. Light sheet fluorescence microscopy (LSFM) is a recently-developed technique
Baskin, Laurence+9 more
core
Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring. [PDF]
The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously ...
Chen, Ching+17 more
core +1 more source
Introducción. El diagnóstico de la leptospirosis es difícil debido al amplio espectro de síntomas clínicos que presenta. Varias pruebas diagnósticas serológicas se han desarrollado, pero su aplicación y utilidad en Colombia no han sido determinadas ...
Piedad Agudelo Flórez+2 more
doaj +1 more source
Development of Optical Biosensor Technologies for Cardiac Troponin Recognition [PDF]
Acute myocardial infarction (AMI) is the leading cause of death among cardiovascular diseases. Among the numerous attempts to develop coronary marker concepts into clinical strategies, cardiac troponin is known as a specific marker for coronary events ...
Abdolrahim, Mojgan+5 more
core +1 more source
Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. [PDF]
Protein synthesis is highly regulated throughout nervous system development, plasticity and regeneration. However, tracking the distributions of specific new protein species has not been possible in living neurons or at the ultrastructural level ...
Butko, Margaret T+9 more
core +1 more source
Arrays of Nano-Electromechanical Biosensors Functionalized by Microcontact Printing [PDF]
The biofunctionalization of nanoelectromechanical structures is critical for the development of new classes of biosensors displaying improved performances and higher-level of integration. We propose a modified microcontact printing method for the functionalization and passivation of large arrays of nanocantilevers in a single, self-aligned step.
arxiv +1 more source
Indirect Fluorescent Antibody Techniques for Demonstration of Trout Viruses and Corresponding Antibody [PDF]
Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT ...
openaire +3 more sources
Fluorescent antibody detection of microorganisms in terrestrial environments [PDF]
The fluorescent antibody technique and its use in direct microscopic examination of the soil is discussed. Feasibility analyses were made to determine if the method could be used to simultaneously observe and recognize microorganisms in the soil.
Schmidt, E. L.
core +1 more source
Patterns of gene expression in schistosomes: localization by whole mount in situ hybridization [PDF]
rom the identification of genes to the characterization of their functions and interactions. Developmental biologists have long used whole mount in situ hybridization (WISH) to determine gene expression patterns, as a vital tool for formulating and ...
Davis+7 more
core +1 more source