Results 91 to 100 of about 513,181 (285)

An open source LABVIEW platform for simulating image series of fluorescent microtubules in gliding assays [PDF]

open access: yes, 2010
We describe an open-source LabVIEW software platform for generating simulated images of microtubules in gliding motility assays.
Lawrence J. Herskowitz, Steven J. Koch
core   +2 more sources

Studies on Fluorescent Dyes. II

open access: yesJournal of Synthetic Organic Chemistry, Japan, 1961
Syntheses and properties of bistriazinylaminostilbene derivatives have been investigated in order to select excellent fluorescent dyes for practical use. In the synthesic experiment, change of combinations of substitution groups on the triazine ring has been made and the derivatives thus obtained were tested on their solubility, acid-resistant ...
openaire   +3 more sources

Heterobifunctional Dyes: Highly Fluorescent Linkers Based on Cyanine Dyes

open access: yesChemistryOpen, 2017
AbstractHerein, we present a new synthetic route to cyanine‐based heterobifunctional dyes and their application as fluorescent linkers between polymers and biomolecules. The synthesized compounds, designed in the visible spectral range, are equipped with two different reactive groups for highly selective conjugation under physiological conditions.
Wycisk, Virginia   +6 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Brewing yeast viability measured using a novel fluorescent dye and image cytometer

open access: yesBiotechnology & Biotechnological Equipment, 2019
This study presents an automated, image-based cytometry method for determination of total counts and viability of yeast cells by using a newly synthesized DNA fluorescent dye PO-TEDM-1 and a new Easycounter YC instrument.
Milka Atanasova   +5 more
doaj   +1 more source

Dye-Doped Fluorescent Silica Nanoparticles for Live Cell and In Vivo Bioimaging

open access: yesNanomaterials, 2016
The need for novel design strategies for fluorescent nanomaterials to improve our understanding of biological activities at the molecular level is increasing rapidly.
Wen-Han Zhang   +2 more
doaj   +1 more source

The Relation between Packing Effects and Solid State Fluorescence of Dyes [PDF]

open access: yes, 1991
The solid state fluorescence of diketopyrrolopyrrole dyes and perylene-3,4:9,10- tetracarboxylic bisimides with alkyl substituents are investigated and compared with noncovalent interactions.
Aubert   +25 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Methods to calibrate and scale axial distances in confocal microscopy as a function of refractive index

open access: yes, 2014
Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration. However, axial distances can be distorted by both the point spread function and by a refractive-index mismatch ...
Besseling, Thijs Herman   +2 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

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