Results 11 to 20 of about 223,239 (351)

A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function

open access: yesBiochemistry and Biophysics Reports, 2020
Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function.
Christopher J. Perry   +8 more
doaj   +1 more source

Research progress of near-infrared fluorescence probes based on indole heptamethine cyanine dyes in vivo and in vitro

open access: yesBMC Chemistry, 2020
Near-infrared (NIR) fluorescence imaging is a noninvasive technique that provides numerous advantages for the real-time in vivo monitoring of biological information in living subjects without the use of ionizing radiation. Near-infrared fluorescent (NIRF)
Chunlong Sun   +4 more
doaj   +1 more source

Investigating fluorescent dyes in fluorescence-assisted screenings [PDF]

open access: yesChem. Commun., 2014
Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads.
Jee, Joo-Eun   +8 more
openaire   +3 more sources

Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.

open access: yesPLoS ONE, 2014
To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ...
Yoko Hayashi-Takanaka   +4 more
doaj   +1 more source

Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy. [PDF]

open access: yesPLoS ONE, 2012
Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy.
Kazuo Yamagata   +8 more
doaj   +1 more source

Fluorescent Probes for STED Optical Nanoscopy

open access: yesNanomaterials, 2021
Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong   +2 more
doaj   +1 more source

Modeling the uptake of fluorescent molecules into 3D cellular spheroids

open access: yesNonlinear Analysis, 2019
Three mathematical models were developed to analyze the dynamics of fluorescent dyes penetration into 3D cellular spheroids. Two fluorescent dyes were chosen to verify mathematical models: rhodamine 6G (R6G) as a small molecule, which can freely ...
Rokas Astrauskas   +4 more
doaj   +1 more source

Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging

open access: yesSmart Molecules, 2023
Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.
Dilizhatai Saimi, Zhixing Chen
doaj   +1 more source

Erasable Optical Fluorescent Data Storage (EOFS) [PDF]

open access: yes, 1991
A novel principle for optical storage is described which is based on organic fluorescent dyes. The information carrier is the lattice of molecular crystals and the information is read out by solid state fluorescence.
Langhals, Heinz
core   +1 more source

Novel application of low pH-dependent fluorescent dyes to examine colitis

open access: yesBMC Gastroenterology, 2010
Background Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and ...
Watanabe Osamu   +3 more
doaj   +1 more source

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