Results 21 to 30 of about 102,467 (263)

Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy. [PDF]

open access: yesPLoS ONE, 2012
Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy.
Kazuo Yamagata   +8 more
doaj   +1 more source

Fluorescent Probes for STED Optical Nanoscopy

open access: yesNanomaterials, 2021
Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong   +2 more
doaj   +1 more source

Modeling the uptake of fluorescent molecules into 3D cellular spheroids

open access: yesNonlinear Analysis, 2019
Three mathematical models were developed to analyze the dynamics of fluorescent dyes penetration into 3D cellular spheroids. Two fluorescent dyes were chosen to verify mathematical models: rhodamine 6G (R6G) as a small molecule, which can freely ...
Rokas Astrauskas   +4 more
doaj   +1 more source

Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging

open access: yesSmart Molecules, 2023
Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.
Dilizhatai Saimi, Zhixing Chen
doaj   +1 more source

Novel application of low pH-dependent fluorescent dyes to examine colitis

open access: yesBMC Gastroenterology, 2010
Background Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and ...
Watanabe Osamu   +3 more
doaj   +1 more source

A Study on the Structure, Optical Properties and Cellular Localization of Novel 1,3-Benzothiazole-Substituted BODIPYs

open access: yesColorants, 2023
A library of seven novel 1,3-benzothiazole-substituted BODIPY derivatives with tunable optical properties was synthesized. The new fluorescent dyes exhibited bathochromically shifted absorptions (up to 670 nm) and emissions centered in the red and near ...
Olga Kirkilessi   +5 more
doaj   +1 more source

Abstract P-10: Solvatochromic Fluorescent Dyes Tested for Spectroscopic Measurements of Protein Conformational Dynamics [PDF]

open access: yesInternational Journal of Biomedicine, 2021
Background: Recoverin is a 23 kDa protein, belonging to the superfamily of EF-hand Ca2+-binding proteins. One of the functions of recoverin is to regulate the activity of the rhodopsin kinase GRK1, which regulates the activity of rhodopsin.
Anatolii Belousov   +6 more
doaj   +1 more source

Evaluation of the diet of a carabid predator using fluorescent marking of prey

open access: yesEuropean Journal of Entomology, 2015
We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader.
Jan OKROUHLIK, Pavel FOLTAN
doaj   +1 more source

Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]

open access: yesPLoS ONE, 2014
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan   +7 more
doaj   +1 more source

Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis

open access: yesAnimal Cells and Systems, 2022
Tissue clearing for 3-dimensional (3D) imaging is increasingly utilized in many biomedical applications, including the pathological examination of human biopsy specimens.
Hyun Jung Kim   +3 more
doaj   +1 more source

Home - About - Disclaimer - Privacy