Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan +7 more
doaj +1 more source
Enhancement of fluorescent properties of near-infrared dyes using clickable oligoglycerol dendrons [PDF]
Near-infrared (NIR) fluorescent dyes are gaining increased attention due to their potential to serve as molecular probes for in vivo imaging. Here, we demonstrate that oligoglycerol dendrons effectively enhance the fluorescence properties of an NIR dye ...
Haag, Rainer +6 more
core +1 more source
Confocal and multiphoton imaging of intracellular Ca2+ [PDF]
This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca<sup>2+</sup> within living cells. In particular, the excitation spectra of popular fluorescent
Bers +42 more
core +1 more source
Staining and peeling of the internal limiting membrane using a fluorescent dye (Rhodamine 6 G) [PDF]
Aim: To assess whether low concentrations of a fluorescent dye such as Rhodamine 6G would help the unaided human eye visualise the vitreous and the internal limiting membrane (ILM) under standard halogen illumination.Material/methods: The UV/Vis ...
Haritoglou, Christos +6 more
core +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
core +1 more source
Fate mapping identifies the origin of SHF/AHF progenitors in the chick primitive streak [PDF]
Heart development depends on the spatio-temporally regulated contribution of progenitor cells from the primary, secondary and anterior heart fields. Primary heart field (PHF) cells are first recruited to form a linear heart tube; later, they contribute ...
Camp, E. +2 more
core +6 more sources
Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis
Tissue clearing for 3-dimensional (3D) imaging is increasingly utilized in many biomedical applications, including the pathological examination of human biopsy specimens.
Hyun Jung Kim +3 more
doaj +1 more source
A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage
In vivo near-infrared (NIR) photoacoustic imaging (PAI) studies using novel contrast agents require validation, often via fluorescence imaging. Bioconjugation of NIR dyes to proteins is a versatile platform to obtain contrast agents for specific ...
Kirsten Cardinell +7 more
doaj +1 more source
A two-state model of twisted intramolecular chargetransfer in monomethine dyes [PDF]
A two-state model Hamiltonian is proposed to model the coupling of twisting displacements to charge-transfer behavior in the ground and excited states of a general monomethine dye molecule.
McKenzie, Ross H., Olsen, Seth
core +1 more source
Proanthocyanidins (PAs) are polymeric phenolic compounds found in plants and used in many industrial applications. Despite strong evidence of herbivore and pathogen resistance-related properties of PAs, their in planta function is not fully understood ...
Jamil Chowdhury +5 more
doaj +1 more source

