Results 291 to 300 of about 154,815 (352)

Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer

Advanced Science, EarlyView.
In this work, a detection method is presented for the activity of plasma DNA methyltransferase 1 (DNMT1), namely DNMT1 Identification by Variable Activity (DIVA). DIVA can detect plasma DNMT1 at levels as low as 10−7 U mL−1, also it successfully evaluated tumor burden and predicted the neoadjuvant therapy responses of breast cancer patients.
Yingran Wang, Guozhi Zhang, Zhizhao Zhang, Mengsi Zhang, Jiao Chen, Ke Wang, Lu Liu, Jing Bao, Ming Chen, Xiaowei Qi, Mingxuan Gao   +10 more
wiley   +1 more source

A MXene‐Based Nanothermal Knife Inhibits Aggresome‐Mediated Persister Formation for Preventing Dental Caries

Advanced Science, EarlyView.
Early childhood caries (ECC) involves cariogenic persisters that evade antimicrobial treatment. Persisters achieve this by downregulating heat shock proteins (HSPs) and formation of protein aggregates. Ti3C2 MXene‐based photothermal therapy (PTT) acts as a “nanothermal knife,” disrupting membranes, upregulating HSPs, inhibits aggregates, prevents ...
Yinyin Zhang, Leilei Yang, Jing jiao, Wenshuai Li, Sen Lin, Xianlong Zong, Haoyang Qin, Danfeng Liu, Rui Li   +8 more
wiley   +1 more source

Aligned Conductive Magnetic Nanofibers with Directional Magnetic Field Stimulation Promotes Peripheral Nerve Regeneration

Advanced Science, EarlyView.
Peripheral nerve injury necessitates alternatives to autografts. This study combines magnetic nanoparticles, oriented PCL fibers, and Ppy to create a conductive, magnetically active scaffold. In vitro and in vivo experiments demonstrated enhanced downstream pathways of calcium signaling, as revealed by transcriptome analysis.
Zheyuan Fan, Wei Yu, Xinggui Wen, Xiangdong Ding, Xiang Li   +4 more
wiley   +1 more source

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Fluorescence In Situ Hybridization

2021
Fluorescence in situ hybridization (FISH) is a method of using fluorescent probes to detect specific nucleic acid sequences within cells and their location. It can be used to detect a variety of cytogenetic variations, including chromosomal deletions, amplification, and translocation.
Min Hu, Weimin Wu
openaire   +3 more sources

Fluorescence In Situ Hybridization

2003
In situ hybridization describes the annealing of a labeled nucleic acid to complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the location of the probe.
Rachel L. Ostroff, Barbara A. Tate
openaire   +6 more sources

Fluorescent In Situ Hybridization

2003
Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.
Elaine K. Green, Sara A. Dyer
openaire   +3 more sources

Fluorescence In Situ Hybridization

1999
Within the past decade, the cytogenetics laboratory has witnessed a major advance in diagnostic and prognostic capability with the advent of molecular cytogenetics. The product of a combination of cytogenetics and molecular biology, the technique of fluorescence in situ hybridization (FISH), has increased the resolution and application of traditional ...
Gesa Schwanitz, Regine Schubert
openaire   +4 more sources

Fluorescence In Situ Hybridization

Clinics in Laboratory Medicine, 2011
This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
openaire   +2 more sources

Fluorescence in situ hybridization

Blood Reviews, 1993
Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
openaire   +2 more sources

An Introduction to Fluorescence in situ Hybridization in Microorganisms

2021
Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for quantification and diversity assessment of microorganisms and, the clinical, for the rapid
Almeida, Carina, Azevedo, Nuno F.
openaire   +4 more sources