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Fluorescence In situ Hybridization
1996During the last decade, pathology has progressed remarkably with the incorporation of molecular techniques into the arena of diagnostics. Fluorescence in situ hybridization (FISH) is a molecular histopathological technique that can be readily used to identify DNA or RNA abnormalities at the cellular level with the use of an epifluorescence microscope ...
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Fluorescent In Situ Hybridization
2001Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Fluorescence in situ hybridization
2007Princip hibridizacijskih tehnika in situ počiva na komplementarnom vezanju obilježene sonde sa specifičnim odsječkom DNA na citogenetskom preparatu, što omogućava izravno utvrđivanje i lokalizaciju određenih sljedova DNA na kromosomu. Stoga ove metode imaju mnoge primjene u istraživanju i dijagnostici (npr.
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Standard Fluorescence in situ Hybridization Procedures [PDF]
, 2015 Fluorescence in situ hybridization (FISH) allows nucleic acid sequences to be visualized inside fi xed cells or chromosomes. The method, based on the principle that nucleic acids can denature and renature, allows the detection of a known nucleotide sequence (probe), in a cell/chromosome, thanks to its ability to hybridize the complementary nucleic-acid
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Fluorescence in situ Hybridization
2017Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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Fluorescence in situ hybridization
1997Abstract In situ hybridization enables the visualization of a probe of interest within a cytological context. For the purposes of gene mapping, the probe is usually a particular segment of cloned DNA which is labelled to allow detection. The target is normally a preparation of chromosomal DNA on a microscope slide.
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Quantitative Fluorescence In Situ Hybridization (QFISH)
2016Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in current molecular cytogenetic and cancer research. This is a unique technique that can be used for chromosomal DNA analysis in all cell types, at all stages of the cell cycle, and at molecular resolution.
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