Multiple fluorescence in situ hybridization [PDF]
AbstractA method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue.This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation ...
M. van der Ploeg+6 more
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Dense transcript profiling in single cells by image correlation decoding [PDF]
Sequential barcoded fluorescent in situ hybridization (seqFISH) allows large numbers of molecular species to be accurately detected in single cells, but multiplexing is limited by the density of barcoded objects. We present correlation FISH (corrFISH), a
A Raj+22 more
core +1 more source
Effect of cycle time and airflow in biological nitrogen removal from poultry slaughterhouse wastewater using sequencing batch reactor [PDF]
This study aimed to evaluate the influence of airflow (0.25, 0.50 and 0.75 L.L-1.min-1) and cycle time (10.45 h, 14.25 h and 17.35 h) on a sequencing batch reactor (SBR) performance in promoting nitrification and denitrification of poultry slaughterhouse
Carla L. Lopes+5 more
doaj +2 more sources
A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M.+4 more
core +1 more source
Exclusive neuronal expression of SUCLA2 in the human brain [PDF]
SUCLA2 encodes the ATP-forming subunit (A-SUCL-) of succinyl-CoA ligase, an enzyme of the citric acid cycle. Mutations in SUCLA2 lead to a mitochondrial disorder manifesting as encephalomyopathy with dystonia, deafness and lesions in the basal ganglia ...
A Kowluru+55 more
core +2 more sources
Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes [PDF]
Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging.
Avendano, M.+17 more
core +1 more source
Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
doaj
Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes [PDF]
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines ...
A.H.N. Hopman+64 more
core +1 more source
Fluorescent in Situ Hybridization
Abstract not availableBangladesh J Med Biochem 2017; 10(1): 3 ...
S Akhter, HB Asad, Z Aziz
openaire +3 more sources
Pericentromeric organization at the fusion point of mouse Robertsonian translocation chromosomes [PDF]
In mammals, Robertsonian (Rb) translocation (the joining of two telo/acrocentric chromosomes at their centromere to form a metacentric) is the most effective process in chromosomal evolution leading to speciation; its occurrence also affects human health
Capanna, E.+5 more
core +3 more sources