Results 31 to 40 of about 340,246 (317)
Improved Affinity: A Customized Fluorescent Probe for the Rapid Detection of Butyrylcholinesterase
ChemosensorsThis article presents the distinctive butyrylcholinesterase (BChE) fluorescent probe P5, designed via a targeting-site method. This method was proposed to enhance the affinity of the probe for BChE by targeting the peripheral anionic site (PAS) of BChE ...
Wei Wang +6 more
doaj +1 more source
Frontiers in Chemistry, 2019 We synthesized novel PET (photoinduced electron transfer)-type fluorescence glucose probe 1 [(4-(anthracen-2-yl-carbamoyl)-3-fluorophenyl)boronic acid], which has a phenylboronic acid (PBA) moiety as the recognition site and anthracene as the fluorescent
Ko Sugita +8 more
doaj +1 more source
Fluorescent Proteins as Proteomic Probes [PDF]
Molecular & Cellular Proteomics, 2005 Protein complexes mediate the majority of cellular processes. Knowledge of the localization and composition of such complexes provides key insights into their functions. Although green fluorescent protein (GFP) has been widely applied for in vivo visualization of proteins, it has been relatively little used as a tool for the isolation of protein ...
Ileana M, Cristea +3 more
openaire +2 more sources
A ratiometric fluorescent probe for rapid and specific detection of hypochlorite
, 2023 Hypochlorite (ClO−), an important reactive oxygen species, plays a crucial role in vitro and vivo. In this work, a ratiometric fluorescent probe (TPAM) was designed and synthesized for sensing ClO− based on substituted triphenylamine and malononitrile ...
Ben Zhong, Tang +5 more
core +1 more source
THE USE OF FLUORESCENT PROBES IN IMMUNOCHEMISTRY* [PDF]
Photochemistry and Photobiology, 1990 Abstract—The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined.
R S, Davidson, M M, Hilchenbach
openaire +2 more sources
A near-infrared fluorescent probe for hydrogen sulfide in living cells
, 2013 Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. However, near-infrared fluorescent probes for H2S are still rare.
Liu, Huiying +6 more
core +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
, 2012 We have described a turn-on near-infrared fluorescent probe Cy-NO2 based on nitro group reduction for intracellular H2S detection. The probe employs cyanine dye as a fluorophore, and is equipped with a nitro group as a fluorescent modulator.
Yu, Fabiao +5 more
core +1 more source
A fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells
, 2022 Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells.
Megan, Wright +2 more
core +1 more source
Lessons in Organic Fluorescent Probe Discovery [PDF]
, 2021 Fluorescent probes have gained profound use in biotechnology, drug discovery, medical diagnostics, molecular and cell biology. The development of methods for the translation of fluorophores into fluorescent probes continues to be a robust field for ...
Maslivetc, Vladimir +10 more
core +1 more source