Results 51 to 60 of about 340,246 (317)

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Theoretical insights into a turn-on fluorescence probe based on naphthalimide for peroxynitrite detection

Heliyon
Compared with other reactive oxygen species, peroxynitrite (ONOO−) has diversified reactions and transformations in organisms, and its specific action mechanism is not very clear. The study of reactive oxygen species is of great significance in the field
He Huang, Zhongfu Zou, Yongjin Peng
doaj   +1 more source

A Schiff Base Fluorescence Enhancement Probe for Fe(III) and Its Sensing Applications in Cancer Cells

Sensors, 2019
We report a new Schiff base fluorescent probe which senses ferric ion, Fe(III), with a significant fluorescence enhancement response. The probe showed high sensitivity (0.8 ppb), and fast response time (<10 s) of Fe(III) in aqueous media. In addition,
Na Hee Kim, Junho Lee, Sungnam Park, Junyang Jung, Dokyoung Kim   +4 more
doaj   +1 more source

Fluorescent probe immobilization into enzyme molecules [PDF]

Компьютерные исследования и моделирование, 2013
The results of the experimental and theoretical researches of kinetics of erythrosine penetration into collagenase molecules have represented in this paper.
Valeriya Sergeevna Maryakhina, Vyacheslav Vasilevich Gunkov   +1 more
doaj   +1 more source

Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn2+ in the Golgi apparatus in live cells

STAR Protocols, 2021
Summary: Quantitative analysis using a turn-on fluorescent probe is inherently difficult due to the dependency of the fluorescence intensity on the probe concentration.
Toshiyuki Kowada, Tomomi Watanabe, Rong Liu, Shin Mizukami   +3 more
doaj   +1 more source

Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

, 2018
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe.
Jean-Christophe, Cintrat, Sébastien, Pomel, Raphael, LABRUERE, Elodie, Jagu, Philippe M., Loiseau, stéphanie, Pethe   +5 more
core   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Fluorescent image-guided surgery in breast cancer by intravenous application of a quenched fluorescence activity-based probe for cysteine cathepsins in a syngeneic mouse model

EJNMMI Research, 2020
Purpose The reoperation rate for breast-conserving surgery is as high as 15–30% due to residual tumor in the surgical cavity after surgery. In vivo tumor-targeted optical molecular imaging may serve as a red-flag technique to improve intraoperative ...
Frans V. Suurs, Si-Qi Qiu, Joshua J. Yim, Carolien P. Schröder, Hetty Timmer-Bosscha, Eric S. Bensen, John T. Santini, Elisabeth G. E. de Vries, Matthew Bogyo, Gooitzen M. van Dam   +9 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe

Sensors, 2018
This study presents chemical modification of a Rhodamine B (RhB) sensor probe by ethylenediamine (EDA), and investigation of its spectral as well as sensor properties to the various metals. The synthesised N-(Rhodamine-B)-lactam-ethylenediamine (RhB-EDA)
Eva Soršak, Julija Volmajer Valh, Špela Korent Urek, Aleksandra Lobnik   +3 more
doaj   +1 more source