Results 51 to 60 of about 1,158,324 (307)

A Schiff Base Fluorescence Enhancement Probe for Fe(III) and Its Sensing Applications in Cancer Cells

Sensors, 2019
We report a new Schiff base fluorescent probe which senses ferric ion, Fe(III), with a significant fluorescence enhancement response. The probe showed high sensitivity (0.8 ppb), and fast response time (<10 s) of Fe(III) in aqueous media. In addition,
Na Hee Kim, Junho Lee, Sungnam Park, Junyang Jung, Dokyoung Kim   +4 more
doaj   +1 more source

Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn2+ in the Golgi apparatus in live cells

STAR Protocols, 2021
Summary: Quantitative analysis using a turn-on fluorescent probe is inherently difficult due to the dependency of the fluorescence intensity on the probe concentration.
Toshiyuki Kowada, Tomomi Watanabe, Rong Liu, Shin Mizukami   +3 more
doaj   +1 more source

A two-photon fluorescent probe for imaging hydrogen sulfide in living cells

, 2013
Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged ...
Zou, Chunyan, Zhang, Xinfu, Qiao, Qinglong, Xu, Zhaochao, Jingnan Cui, Liu, Tianyu, Cui, Jingnan, Feng, Lei, 徐兆超   +8 more
core   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Fluorescent image-guided surgery in breast cancer by intravenous application of a quenched fluorescence activity-based probe for cysteine cathepsins in a syngeneic mouse model

EJNMMI Research, 2020
Purpose The reoperation rate for breast-conserving surgery is as high as 15–30% due to residual tumor in the surgical cavity after surgery. In vivo tumor-targeted optical molecular imaging may serve as a red-flag technique to improve intraoperative ...
Frans V. Suurs, Si-Qi Qiu, Joshua J. Yim, Carolien P. Schröder, Hetty Timmer-Bosscha, Eric S. Bensen, John T. Santini, Elisabeth G. E. de Vries, Matthew Bogyo, Gooitzen M. van Dam   +9 more
doaj   +1 more source

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery [PDF]

, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Bhavyashree Suresh, Ferhan Ayaydin, Bokros, Attila, Eszter Molnár, Peksel, Begüm, Ágnes Medgyesi, Liliána Fehér, Pradeep Kumar Reddy Cingaram, Puskás, László G., Soujanya Kuntam, Attila Bokros, Begüm Peksel, Gabriella Fábián, Molnár, Eszter, László G. Puskás, Kuntam, Soujanya, Liliána Z. Fehér, Fábián, Gabriella, László Puskás, Cingaram, Pradeep Kumar Reddy, Pradeep Cingaram   +20 more
core   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Fluorescent probe immobilization into enzyme molecules [PDF]

Компьютерные исследования и моделирование, 2013
The results of the experimental and theoretical researches of kinetics of erythrosine penetration into collagenase molecules have represented in this paper.
Valeriya Sergeevna Maryakhina, Vyacheslav Vasilevich Gunkov   +1 more
doaj   +1 more source

A DIE responsive NIR-fluorescent cell membrane probe

, 2018
It is challenging to achieve selective off to on modulation of the emissive state of a fluorophore within a complex and heterogeneous cellular environment.
Cheung, Shane, Durán Sampedro, Gonzalo, Chen, Zhi-Long, O'Shea, Donal, Cahill, Ronan, Wu, Dan   +5 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source