Results 121 to 130 of about 930,400 (311)

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Gate‐Controlled Photoresponse in an Individual Single‐Walled Carbon Nanotube Modified with a Fluorescent Protein

Advanced Electronic Materials
Bionanohybrids of carbon nanotubes and fluorescent proteins (FPs) are a promising class of materials for optoelectronic applications. Understanding and controlling the charge transport mechanism between FPs and carbon nanotubes are critical to achieving ...
Anastasiia S. Kudriavtseva, Nikita P. Nekrasov, Dmitry V. Krasnikov, Albert G. Nasibulin, Alexey M. Bogdanov, Ivan Bobrinetskiy   +5 more
doaj   +1 more source

Green Fluorescent Protein (GFP) color reporter gene visualizes parvovirus B19 Non-structural Segment 1 (NS1) transfected endothelial modification

, 2012
Background: Human Parvovirus B19 (PVB19) has been associated with myocarditis putative due to endothelial infection. Whether PVB19 infects endothelial cells and causes a modification of endothelial function and inflammation and, thus, disturbance of ...
Seizer, Peter, Seizer Peter, Paul, Angela, Thomas Wurster, Botnar Rene M., Schönberger, Tanja, Stellos, Konstantinos L., Gawaz, Meinrad Paul, Tanja Schönberger, Gawaz Meinrad, Schuster Andreas, Gawaz, Meinrad, Botnar, Rene M.; id_orcid, Stellos, Konstantinos, Paul Angela, Boris Bigalke, Stellos Konstantinos, Pölzelbauer Catharina, Schönberger Tanja, Poelzelbauer, Catharina, Wurster, Thomas Heinrich, Angela Paul, Konstantinos Stellos, Meinrad Gawaz, Bigalke, Boris, Bigalke Boris, Botnar, Rene Michael, Rene M Botnar, Andreas Schuster, Wurster Thomas, Peter Seizer, Schuster, Andreas, Wurster, Thomas, Schoenberger, Tanja, Pölzelbauer, Catharina, Catharina Pölzelbauer   +35 more
core   +1 more source

Network divergence analysis identifies adaptive gene modules and two orthogonal vulnerability axes in pancreatic cancer

Molecular Oncology, EarlyView.
Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson, Lyanne Delgado‐Coka, Natalia Marchenko, Luisa F. Escobar‐Hoyos, Kenneth R. Shroyer, Alisa Yurovsky, Trey Ideker, Gábor Balázsi, Thomas MacCarthy, Scott Powers   +9 more
wiley   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Fluorescence resonance energy transfer studies of protein interactions

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
core  

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)

Indonesian Journal of Biotechnology, 2015
The application of fluorescent proteins as expression markers and protein fusion partners has provedimmensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj  

Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker

, 2009
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X., Li, W., Li, J., Zhang, N., Liang, X., Zhang, X., Gong, P., Ma, G., Ryan, U.M.   +8 more
core  

Ratiometric detection of Zn(ll) using chelating fluorescent protein chimeras

, 2007
Fluorescent indicators for the real-time imaging of small mols. or metal ions in living cells are invaluable tools for understanding their physiol. function.
Merkx, M., Appelhof, M.A.M., Evers, T.H., Meijer, E.W., Graaf-Heuvelmans, de, P.T.H.M.   +4 more
core   +1 more source