Results 101 to 110 of about 930,400 (311)

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [PDF]

, 2013
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby.
Looger, Loren L, Tolö, Johan, Michael B Orger, Leon eLagnado, Tian, Lin, Pulver, Stefan R., Marvin, Jonathan S, Gottschalk, Alexander, Lin eTian, Leon Lagnado, Carreras Calderón, Nicole, Jasper eAkerboom, Kristen E Severi, Eric R Schreiter, Kristen E. Severi, Ronak ePatel, Alexander Gottschalk, Matthias ePrigge, Nicole Carreras Calderón, Kim, Douglas S, Lin Tian, Cornelia I Bargmann, Calderon, Nicole Carreras, Marvin, Jonathan S., Sebastian eKügler, Jasper Akerboom, Douglas S Kim, Douglas S. Kim, Orger, Michael B, Tsai-Wen eChen, Macklin, John J., Sebastian Wabnig, Tsai-Wen Chen, Elisabeth Fischer, Alexander eGottschalk, Elisabeth eFischer, Sarkisyan, Karen S, Marvin, Jonathan, Schreiter, Eric, Matthias Prigge, Wardill, Trevor J, Andrew eGordus, John J Macklin, Schüler, Christina, Trevor J. Wardill, Ronak Patel, Sebastian eWabnig, Severi, Kristen E., Nicole eCarreras Calderón, Severi, Kristen E, Loren L. Looger, Trevor J Wardill, Karen S. Sarkisyan, Christina Schüler, Fischer, Elisabeth, Patel, Ronak, Hegemann, Peter, Stefan R Pulver, Pulver, Stefan R, Kügler, Sebastian, Wardill, Trevor, Eric R. Schreiter, Loren L Looger, Wabnig, Sebastian, Jonathan S. Marvin, Johan Tolö, Sebastian Kügler, Schreiter, Eric R., Patel, Ronak Bhailal, Cornelia I. Bargmann, Sarkisyan, Karen S., Schueler, Christina, John J. Macklin, Andrew Gordus, Karen S Sarkisyan, Chen, Tsai-Wen, Bargmann, Cornelia I., Jonathan S Marvin, Looger, Loren L., Kim, Douglas S., Orger, Michael B., Schreiter, Eric R, Lagnado, Leon, Johan eTolö, Peter Hegemann, Orger, Michael, Prigge, Matthias, Bargmann, Cornelia I, Gordus, Andrew, Christina eSchüler, Stefan R. Pulver, Michael B. Orger, Wardill, Trevor J., Severi, Kristen, Bargmann, Cornelia, Peter eHegemann, Akerboom, Jasper, Macklin, John J   +97 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare and diagnostics, and research into their biological functions is rapidly increasing.
Maria S. Panagopoulou, Alastair W. Wark, David J S Birch, Christopher D. Gregory   +3 more
doaj   +1 more source

Murine leukemia virus (MLV) replication monitored with fluorescent proteins

, 2006
Background: Cancer gene therapy will benefit from vectors that are able to replicate in tumor tissue and cause a bystander effect. Replication-competent murine leukemia virus (MLV) has been described to have potential as cancer therapeutics, however, MLV
Erlwein, Otto, Bittner, Alexandra, Sliva, Katja, Schnierle, Barbara   +3 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides

Pharmaceuticals, 2011
Many genetic and infectious diseases can be targeted at the RNA level as RNA is more accessible than DNA. We seek to develop new approaches for detection and tracking RNA in live cells, which is necessary for RNA-based diagnostics and therapy.
Charles R. Cantor, Natalia E. Broude, Paul Toran, Hung-Wei Yiu, Vadim V. Demidov   +4 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia, Webb, Martin R, Mashanov, Gregory I, Chisty, Liisa T, Toseland, Christopher P., Dillingham, Mark S, Toseland, Christopher P, Molloy, Justin E   +7 more
core   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy

Sensors, 2012
The ability to colonize the host plants’ rhizospheres is a crucial feature to study in the case of Plant Growth Promoting Rhizobacteria (PGPRs) with potential agricultural applications.
Sylwia Jafra, Michal Rychlowski, Mariusz Bikowski, Dorota Krzyzanowska, Michal Obuchowski   +4 more
doaj   +1 more source