Results 111 to 120 of about 930,400 (311)

α‐Synuclein aggregation landscape from phase separation to neurotoxic intermediates

FEBS Letters, EarlyView.
Alpha‐synuclein aggregation in Parkinson's disease involves a complex landscape of transient intermediates, including oligomers, fibrils and liquid–liquid phase separation (LLPS). A view is emerging in which LLPS maturation into solid‐like condensates may contribute to the formation of neurotoxic species.
Silvia Arino, Giuliana Fusco, Alfonso De Simone   +2 more
wiley   +1 more source

Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes

Frontiers in Cellular and Infection Microbiology, 2019
Numerous methods exist for fluorescently labeling proteins either as direct fusion proteins (GFP, RFP, YFP, etc.—attached to the protein of interest) or utilizing accessory proteins to produce fluorescence (SNAP-tag, CLIP-tag), but the significant ...
Chadwick Hillman, Philip E. Stewart, Martin Strnad, Martin Strnad, Martin Strnad, Hunter Stone, Tregei Starr, Aaron Carmody, Tyler J. Evans, Valentina Carracoi, Jenny Wachter, Patricia A. Rosa   +11 more
doaj   +1 more source

Colorful protein-based fluorescent probes for collagen imaging [PDF]

, 2014
Real-time visualization of collagen is important in studies on tissue formation and remodeling in the research fields of developmental biology and tissue engineering.
Aper, SJA Stijn, Turnhout, MC Mark van, Spreeuwel, ACC Ariane van, Merkx, M., Zon, van der, N.L.L., Rambelje, de la, S.L., Bouten, C.V.C., Bouten, CVC Carlijn, Pieters, PA Pascal, Merkx, M Maarten, Turnhout, van, M.C.; id_orcid, Linden, AJ Ardjan van der, Rambelje, SL de la, Turnhout, van, M.C., Spreeuwel, van, A.C.C., Bouten, C.V.C.; id_orcid, Aper, S.J.A., Zon, NLL van der, van der Linden, A.J., Pieters, P.A.   +19 more
core   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

The feeding tube of cyst nematodes:characterisation of protein exclusion [PDF]

, 2014
Plant parasitic nematodes comprise several groups; the most economically damaging of these are the sedentary endoparasites. Sedentary endoparasitic nematodes are obligate biotrophs and modify host root tissue, using a suite of effector proteins, to ...
Jones, John T., Ault James R., John T Jones, Eves-van den Akker, Sebastian, James R. Ault (515791), Alison E Ashcroft, Jones John T., Urwin, Peter E., Jones, JT, Catherine J Lilley, Eves-van den Akker Sebastian, Eves-van den Akker, S, Catherine J. Lilley (182995), Ashcroft, Alison E., Lilley Catherine J., Sebastian Eves-van den Akker, Urwin Peter E., John T. Jones (207247), Ault, James R., James R Ault, Peter E. Urwin (183000), Ashcroft, Alison E, Lilley, Catherine J., Ault, JR, Alison E. Ashcroft (515792), Ashcroft, AE, Ashcroft Alison E., Sebastian Eves-van den Akker (515790), Lilley, CJ, Urwin, PE, Peter E Urwin   +30 more
core   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae [PDF]

, 2011
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood.
Stephan Gruber, Thon, Maria,, Gruber, Stephan, Attaiech, Laetitia,, Anita Minnen, Minnen, Anita,, Attaiech, L., Attaiech, Laetitia, Thon, Maria, Gruber, S., Gruber, Stephan,, Veening, Jan-Willem,, Laetitia Attaiech, Jan-Willem Veening, Minnen, A., Veening, J. W., Maria Thon, Thon, M., Minnen, Anita, Veening, Jan-Willem   +19 more
core   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene

, 2008
Fluorescent reference strains of bacteria carrying a stable chromosomally integrated single copy of the gfp gene have been developed. A modified version of the gfp gene has been generated by mutagenesis and expressed under the control of the ...
Gibbs, Moreland D., Vesey, Graham, Smith, James J., Pinheiro, Leonardo B., Berquist, Peter L.   +4 more
core   +1 more source