Results 81 to 90 of about 930,400 (311)

Genetically Encoded Calcium Indicators Based on Troponin C and Fluorescent Proteins [PDF]

, 2005
Genetically encoded calcium probes allow the visualization and quantification of intracellular calcium dynamics with great specificity and sensitivity.
Heim, Nicola, Heim, N.
core  

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Express your LOV: an engineered flavoprotein as a reporter for protein expression and purification [PDF]

, 2012
In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function.
Jayde A Gawthorne, Akpunarlieva, S.N., John M. Christie (107410), Beckham, K.S.H., Gabrielsen, M., Katherine S. H. Beckham (107407), Alto, Neal M., Alto, N.M., Christie, J.M., Snezhana N. Akpunarlieva (107403), Jayde A. Gawthorne (107393), Mads Gabrielsen (107416), Reddick, L.E., Akpunarlieva, Snezhana N., Jayde A. Gawthorne, Roe, A.J., Katherine S H Beckham, Christie, John M., Snezhana N. Akpunarlieva, L. Evan Reddick (107398), Beckham, Katherine S. H., Mads Gabrielsen, Andrew J Roe, John M Christie, Gabrielsen, Mads, Gawthorne, J.A., Reddick, L. Evan, Andrew J. Roe, Neal M. Alto (107413), John M. Christie, L. Evan Reddick, Snezhana N Akpunarlieva, Neal M. Alto, Andrew J. Roe (107419), Neal M Alto, Roe, Andrew J., Katherine S. H. Beckham, L Evan Reddick, Gawthorne, Jayde A.   +38 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display

Frontiers in Plant Science, 2017
Plant viruses are suitable as building blocks for nanomaterials and nanoparticles because they are easy to modify and can be expressed and purified using plants or heterologous expression systems.
Juliane Röder, Rainer Fischer, Ulrich Commandeur   +2 more
doaj   +1 more source

Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research

Scientific Reports, 2021
The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research.
Kara L. Schneider, Adam J. M. Wollman, Thomas Nyström, Sviatlana Shashkova   +3 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

PNA-induced assembly of fluorescent proteins using DNA as a framework

, 2013
Controlled alignment of proteins on molecular frameworks requires the development of facile and orthogonal chemical approaches and molecular scaffolds. In this work, protein−PNA conjugates are brought forward as new chemical components allowing efficient
Gholami, Z., Brunsveld, L., Gholami, Z, Hanley, Q, Hanley, Q., Brunsweld, L, Brunsveld, L.; id_orcid   +6 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source