Bright Dots and Smart Optical Microscopy to Probe Intracellular Events in Single Cells
Frontiers in Bioengineering and Biotechnology, 2019 Probing intracellular events is a key step in developing new biomedical methodologies. Optical microscopy has been one of the best options to observe biological samples at single cell and sub-cellular resolutions.
Hideaki Fujita +5 more
doaj +1 more source
Green Fluorescent Protein: discover, structure, purification, applications, and future prospects
Journal of Scientific Research in Science, 2023 Green fluorescent protein (GFP) Firstly discovered from the jellyfish Aequorea victoriaand it was widely used in biological science as a universal fluorescent tag.
Hend A. Anter +2 more
doaj +1 more source
Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective. [PDF]
, 2017 Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities.
Candas, Demet +7 more
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Live-Cell Imaging of Physiologically Relevant Metal Ions Using Genetically Encoded FRET-Based Probes
Cells, 2019 Essential biochemical reactions and processes within living organisms are coupled to subcellular fluctuations of metal ions. Disturbances in cellular metal ion homeostasis are frequently associated with pathological alterations, including neurotoxicity ...
Helmut Bischof +7 more
doaj +1 more source
Specialized mouse embryonic stem cells for studying vascular development. [PDF]
, 2014 Vascular progenitor cells are desirable in a variety of therapeutic strategies; however, the lineage commitment of endothelial and smooth muscle cell from a common progenitor is not well-understood.
Burns, Andrew B +5 more
core +1 more source
Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression
Cell Structure and Function, 2023 We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence.
Ryoko Ando +3 more
doaj +1 more source
Studies of Single-Molecule Dynamics in Microorganisms [PDF]
, 2019 Fluorescence microscopy is one of the most extensively used techniques in the life sciences. Considering the non-invasive sample preparation, enabling live-cell compliant imaging, and the specific fluorescence labeling, allowing for a specific visualization
Turkowyd, Bartosz Daniel
core +1 more source
Modulation of Fluorescent Protein Chromophores To Detect Protein Aggregation with Turn-On Fluorescence [PDF]
Journal of the American Chemical Society, 2018 We present a fluorogenic method to visualize misfolding and aggregation of a specific protein-of-interest in live cells using structurally modulated fluorescent protein chromophores. Combining photophysical analysis, X-ray crystallography, and theoretical calculation, we show that fluorescence is triggered by inhibition of twisted-intramolecular charge
Yu Liu +14 more
openaire +2 more sources
Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display
Frontiers in Plant Science, 2017 Plant viruses are suitable as building blocks for nanomaterials and nanoparticles because they are easy to modify and can be expressed and purified using plants or heterologous expression systems.
Juliane Röder +2 more
doaj +1 more source
Adenoviral targeting of malignant melanoma for fluorescence-guided surgery prevents recurrence in orthotopic nude-mouse models. [PDF]
, 2015 Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in ...
Bouvet, Michael +7 more
core +1 more source