Results 61 to 70 of about 930,400 (311)

Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)

Indonesian Journal of Biotechnology, 2005
The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos

BioTechniques, 2001
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley, Ann E. Davidson, Stephen C. Ekker   +2 more
doaj   +1 more source

Knock-In Mouse Models to Investigate the Functions of Opioid Receptors in vivo

Frontiers in Cellular Neuroscience, 2022
Due to their low expression levels, complex multi-pass transmembrane structure, and the current lack of highly specific antibodies, the assessment of endogenous G protein-coupled receptors (GPCRs) remains challenging. While most of the research regarding
Jade Degrandmaison, Jade Degrandmaison, Jade Degrandmaison, Samuel Rochon-Haché, Samuel Rochon-Haché, Samuel Rochon-Haché, Jean-Luc Parent, Louis Gendron, Louis Gendron   +8 more
doaj   +1 more source

Screening by coral green fluorescent protein (GFP)-like chromoproteins supports a role in photoprotection of zooxanthellae [PDF]

, 2013
Green fluorescent protein (GFP)-like pigments are responsible for the vivid colouration of many reef-building corals and have been proposed to act as photoprotectants.
Smith, E.G., Salih, A., D'Angelo, Cecilia, Smith, E. G., D’Angelo, C., Wiedenmann, J., Smith, Edward G., Wiedenmann, Jörg, Salih, Anya (R12014), D'Angelo, C.   +9 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Genetically encoded fluorescent biosensors for GPCR research

Frontiers in Cell and Developmental Biology, 2022
G protein-coupled receptors (GPCRs) regulate a wide range of physiological and pathophysiological cellular processes, thus it is important to understand how GPCRs are activated and function in various cellular contexts.
Hyunbin Kim, Hyunbin Kim, In-Yeop Baek, In-Yeop Baek, Jihye Seong, Jihye Seong, Jihye Seong   +6 more
doaj   +1 more source

His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor [PDF]

, 2008
Fluorescent indicators that allow real-time imaging of Zn(II) in living cells are invaluable tools for understanding Zn(II) homeostasis. Genetically encoded sensors based on fluorescence resonance energy transfer between fluorescent protein domains have ...
Meijer, EW Bert, Merkx, M., Appelhof, M.A.M., Evers, T.H., Meijer, E.W., Merkx, M Maarten, Evers, TH Toon, Appelhof, MAM Marieke   +7 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor in Salmonella

Biomolecules, 2020
The bacterial flagellar motor converts the energy of proton flow through the MotA/MotB complex into mechanical works required for motor rotation. The rotational force is generated by electrostatic interactions between the stator protein MotA and the ...
Yusuke V. Morimoto, Keiichi Namba, Tohru Minamino   +2 more
doaj   +1 more source