Results 41 to 50 of about 471,648 (132)
Molecular Systems Biology, 2020 Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies.
Jiegen Wu +4 more
doaj +1 more source
Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases
Frontiers in Molecular Biosciences, 2022 The recently discovered Rhodopsin-cyclases from Chytridiomycota fungi show completely unexpected properties for microbial rhodopsins. These photoreceptors function exclusively as heterodimers, with the two subunits that have very different retinal ...
Matthias Broser
doaj +1 more source
Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research [PDF]
, 2019 Hypertension is ranked as the third cause of disability-adjusted life-years. The percentage of the population suffering from hypertension will continue to increase over the next years.
Schleifenbaum, Johanna, Zhong, Cheng
core +1 more source
Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions
Frontiers in Cellular and Infection Microbiology, 2017 Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined ...
Kendal G. Cooper +4 more
doaj +1 more source
Frontiers in Chemistry, 2021 Single Molecule Localization Microscopy (SMLM) is an imaging method that allows for the visualization of structures smaller than the diffraction limit of light (~200 nm).
Pooja Laxman +3 more
doaj +1 more source
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
core +2 more sources
Fluorescent Probes for STED Optical Nanoscopy
Nanomaterials, 2021 Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong +2 more
doaj +1 more source
Quantitative assessment of fluorescent proteins [PDF]
Nature Methods, 2016 The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight β-barrel structure, which provides the rigidity and chemical ...
Cranfill, P J +9 more
openaire +2 more sources
Biosensors, 2019 In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells.
Kira Erismann-Ebner +2 more
doaj +1 more source
Quantum yield optimized fluorophores for site-specific labeling and super-resolution imaging
, 2011 Single molecule applications, saturated pattern excitation microscopy, or stimulated emission depletion (STED) microscopy demand for bright and highly stable fluorescent dyes1,2.
Choquet, Daniel +6 more
core +3 more sources