Results 41 to 50 of about 930,400 (311)

Optimizing the Protein Fluorescence Reporting System for Somatic Embryogenesis Regeneration Screening and Visual Labeling of Functional Genes in Cotton

Frontiers in Plant Science, 2022
Protein fluorescence reporting systems are of crucial importance to in-depth life science research, providing systematic labeling tools for visualization of microscopic biological activities in vivo and revolutionizing basic research. Cotton somatic cell
Gai-Yuan Hu, Gai-Yuan Hu, Jia-Yi Ma, Jia-Yi Ma, Fen Li, Fen Li, Jing-Ruo Zhao, Jing-Ruo Zhao, Fu-Chun Xu, Fu-Chun Xu, Wen-Wen Yang, Man Yuan, Wei Gao, Wei Gao, Lu Long, Lu Long   +15 more
doaj   +1 more source

Quantitative assessment of fluorescent proteins [PDF]

Nature Methods, 2016
The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight β-barrel structure, which provides the rigidity and chemical ...
Cranfill, P J, Sell, B R, Baird, M A, Allen, J R, Lavagnino, Z, de Gruiter, Martijn, Kremers, Gert-Jan, Davidson, M W, Ustione, A, Piston, D W   +9 more
openaire   +2 more sources

A beta-herpesvirus with fluorescent capsids to study transport in living cells. [PDF]

, 2012
Fluorescent tagging of viral particles by genetic means enables the study of virus dynamics in living cells. However, the study of beta-herpesvirus entry and morphogenesis by this method is currently limited.
Lisa Marcinowski, Marcinowski, Lisa, Ulrich H Koszinowski, Christophe Jung (152124), Jens von Einem, Gaul, Ulrike, Paul Walther, Einem, Jens von, Jens B Bosse, Paul Walther (113867), Ruzsics, Zsolt, Ulrike Gaul, Ulrich H. Koszinowski (152143), Bosse, Jens B., Zsolt Ruzsics (75383), Leonhard Popilka (152101), Leonhard Popilka, Christophe Jung, Jens B. Bosse (138966), Lisa Marcinowski (138962), Jens B. Bosse, Martina Taeglich (152115), Rudolf Bauerfeind (152093), Taeglich, Martina, Koszinowski, Ulrich H., Hannah Striebinger, Zsolt Ruzsics, Ulrike Gaul (3154), Ulrich H. Koszinowski, Jung, Christophe, Hannah Striebinger (152128), Martina Taeglich, Jens von Einem (152134), Bauerfeind, Rudolf, Striebinger, Hannah, Rudolf Bauerfeind, Walther, Paul, Popilka, Leonhard   +37 more
core   +1 more source

Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives

BioTechniques, 2012
Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms.
Yutaka Kodama, Chang-Deng Hu
doaj   +1 more source

Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy

Frontiers in Molecular Biosciences, 2021
We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.
Sangyoon Ko, Sangyoon Ko, Jiwoong Kwon, Sang-Hee Shim, Sang-Hee Shim   +4 more
doaj   +1 more source

Tandem fluorescent protein timers for non-invasive relative protein lifetime measurement in plants [PDF]

, 2019
Targeted protein degradation is an important and pervasive regulatory mechanism in plants, required for perception and response to the environment as well as developmental signalling.
Theodoulou, F. L., Linster, E., Gannon, L., Wirtz, M., Zhang, H., Rundle, C., Leemhuis, W.   +6 more
core   +1 more source

Genetically Encoded Biosensors Based on Fluorescent Proteins

Sensors, 2021
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim, Jeongmin Ju, Hae Nim Lee, Hyeyeon Chun, Jihye Seong   +4 more
doaj   +1 more source

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]

, 2012
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089), Antonia Jack (165084), Sandra B Hake, Patricia Wolf, Pichler Garwin, Pichler, Garwin, Hake, Sandra B., Antonia Jack, Wolf Patricia, Garwin Pichler (165082), Jack Antonia, Wolf, Patricia, Jack, Antonia, Hake Sandra B., Garwin Pichler, Patricia Wolf (165088)   +15 more
core   +1 more source

Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes

Viruses, 2021
The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts.
Aitor Nogales, Michael Schotsaert, Raveen Rathnasinghe, Marta L. DeDiego, Adolfo García-Sastre, Luis Martinez-Sobrido   +5 more
doaj   +1 more source

Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells

Molecular Systems Biology, 2020
Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies.
Jiegen Wu, Xu Han, Haotian Zhai, Tingyu Yang, Yihan Lin   +4 more
doaj   +1 more source