Results 31 to 40 of about 867,854 (260)

Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions

Frontiers in Cellular and Infection Microbiology, 2017
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined ...
Kendal G. Cooper, Audrey Chong, Tregei Starr, Ciaran E. Finn, Olivia Steele-Mortimer   +4 more
doaj   +1 more source

Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells

Molecular Systems Biology, 2020
Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies.
Jiegen Wu, Xu Han, Haotian Zhai, Tingyu Yang, Yihan Lin   +4 more
doaj   +1 more source

Red fluorescent proteins engineered from green fluorescent proteins

Proceedings of the National Academy of Sciences, 2023
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Hiromi Imamura, Shiho Otsubo, Mizuho Nishida, Norihiro Takekawa, Katsumi Imada   +4 more
openaire   +2 more sources

In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics

Biosensors, 2019
In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells.
Kira Erismann-Ebner, Anne Marowsky, Michael Arand   +2 more
doaj   +1 more source

Electrochemical fluorescence switching of enhanced green fluorescent protein

Biosensors and Bioelectronics, 2023
Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y, Fan, S, Webb, JA, Ma, Y, Goyette, J, Chen, X, Gaus, K, Tilley, RD, Gooding, JJ   +8 more
openaire   +3 more sources

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2017
Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen, Hjalte V. Kiefer, Henrik B. Pedersen, Anastasia V. Bochenkova, Lars H. Andersen   +4 more
openaire   +3 more sources

Personalized Zebrafish Models for Fusion‐Positive Pediatric Sarcomas

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Clinical sequencing efforts have revolutionized our approaches to categorizing pediatric cancers in real time. This has dramatically improved our ability to profile pediatric tumors, identify actionable vulnerabilities, and influence clinical care.
Lisa H. Hall, Emma N. Harrison, Genevieve C. Kendall   +2 more
wiley   +1 more source

Knock-In Mouse Models to Investigate the Functions of Opioid Receptors in vivo

Frontiers in Cellular Neuroscience, 2022
Due to their low expression levels, complex multi-pass transmembrane structure, and the current lack of highly specific antibodies, the assessment of endogenous G protein-coupled receptors (GPCRs) remains challenging. While most of the research regarding
Jade Degrandmaison, Jade Degrandmaison, Jade Degrandmaison, Samuel Rochon-Haché, Samuel Rochon-Haché, Samuel Rochon-Haché, Jean-Luc Parent, Louis Gendron, Louis Gendron   +8 more
doaj   +1 more source

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
openaire   +2 more sources

Clinical, Histopathological, and Molecular Characterization of Pediatric MN1::ZNF341‐Associated Cancer

Pediatric Blood &Cancer, EarlyView.
ABSTRACT A lethal round‐cell malignancy with an MN1::ZNF341 fusion has recently been reported in three infants. Here, we describe four further tumors, three in newborns (including monozygotic twins), and one in an adolescent. Detailed clinical, radiological, and histopathological data differentiate these tumors from their main mimics, neuroblastoma and
Thomas R. W. Oliver, Dyanne Rampling, Barbara Walkowiak, Nathaniel D. Anderson, Manas Dave, Henry Lee‐Six, Sadie Baskind, Pooja Balasubramanian, Glenn Anderson, Conor Parks, Toochi Ogbonnah, Emilia R. Robertson, Simon Hannam, Rohan Verma, John C. Achermann, Liina Palm, Neil Sebire, Thomas Jacques, Tom Watson, Carryl Dryden, Karin Straathof, Mette Jorgensen, Olga Slater, Sam Behjati, Sarita Depani, J. Ciaran Hutchinson   +25 more
wiley   +1 more source