Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]
, 2008 The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Andrade, S.M. +4 more
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Frontiers in Plant Science, 2022 Protein fluorescence reporting systems are of crucial importance to in-depth life science research, providing systematic labeling tools for visualization of microscopic biological activities in vivo and revolutionizing basic research. Cotton somatic cell
Gai-Yuan Hu +15 more
doaj +1 more source
Computationally designed libraries of fluorescent proteins evaluated by preservation and diversity of function [PDF]
, 2006 To determine which of seven library design algorithms best introduces new protein function without destroying it altogether, seven combinatorial libraries of green fluorescent protein variants were designed and synthesized.
Bokman +32 more
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Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives
BioTechniques, 2012 Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms.
Yutaka Kodama, Chang-Deng Hu
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Scientific Reports, 2021 The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research.
Kara L. Schneider +3 more
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Dynamin Is Functionally Coupled to Insulin Granule Exocytosis [PDF]
, 2007 The insulin granule integral membrane protein marker phogrin-green fluorescent protein was co-localized with insulin in Min6B1 beta-cell secretory granules but did not undergo plasma membrane translocation following glucose stimulation.
Gaisano, HY +7 more
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Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy
Frontiers in Molecular Biosciences, 2021 We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.
Sangyoon Ko +4 more
doaj +1 more source
Recent advances in the synthesis and application of fluorescent α-amino acids [PDF]
, 2016 Fluorescence spectroscopy has become a powerful technique for probing a range of complex biological processes including enzyme mechanisms and protein-protein interactions. While the application of this technique uses a number of strategies, many of these
Harkiss, Alexander, Sutherland, Andrew
core +1 more source
Genetically Encoded Biosensors Based on Fluorescent Proteins
Sensors, 2021 Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim +4 more
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Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Viruses, 2021 The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts.
Aitor Nogales +5 more
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