Results 21 to 30 of about 471,648 (132)
Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]
Journal of the American Chemical Society, 2017 Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen +4 more
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Electrochemical fluorescence switching of enhanced green fluorescent protein
Biosensors and Bioelectronics, 2023 Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y +8 more
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Fluorescent Protein Approaches in Alpha Herpesvirus Research
Viruses, 2015 In the nearly two decades since the popularization of green fluorescent protein (GFP), fluorescent protein-based methodologies have revolutionized molecular and cell biology, allowing us to literally see biological processes as never before.
Ian B. Hogue +6 more
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Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]
The Journal of Physical Chemistry B, 1999 The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G. +3 more
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Fluorescent Protein-Based Metal Biosensors
Chemosensors, 2023 Fluorescent proteins (FPs) are optical probes that are used to track the functions of genetically encoded target molecules in molecular and cellular biology.
Ki Hyun Nam
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Indonesian Journal of Biotechnology, 2005 The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
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On the Origin of Fluorescence in Bacteriophytochrome Infrared Fluorescent Proteins [PDF]
The Journal of Physical Chemistry B, 2010 Tsien et al. (Science, 2009, 324, 804-807) recently reported the creation of the first infrared fluorescent protein (IFP). It was engineered from bacterial phytochrome by removing the PHY and histidine kinase-related domains, by optimizing the protein to prevent dimerization, and by limiting the biliverdins conformational freedom, especially around its
Alex A, Samma +4 more
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Nature Communications, 2017 Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control ...
Cindy Ast +6 more
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Improved split fluorescent proteins for endogenous protein labeling. [PDF]
, 2017 Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact.
Feng, Siyu +5 more
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Homotransfer FRET Reporters for Live Cell Imaging
Biosensors, 2018 Förster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications.
Nicole E. Snell +6 more
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