Results 21 to 30 of about 867,854 (260)
Frontiers in Plant Science, 2022 Protein fluorescence reporting systems are of crucial importance to in-depth life science research, providing systematic labeling tools for visualization of microscopic biological activities in vivo and revolutionizing basic research. Cotton somatic cell
Gai-Yuan Hu +15 more
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Homotransfer FRET Reporters for Live Cell Imaging
Biosensors, 2018 Förster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications.
Nicole E. Snell +6 more
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Fluorescent protein spectra [PDF]
Journal of Cell Science, 2001 The cloning of the green fluorescent protein (GFP) from the jellyfish Aequoria victoria and its expression in heterologous systems was a significant advance for optical microscopy of living cells ([Chalfie et al., 1994][1]).
G, Patterson, R N, Day, D, Piston
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Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy
Frontiers in Molecular Biosciences, 2021 We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.
Sangyoon Ko +4 more
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Genetically Encoded Biosensors Based on Fluorescent Proteins
Sensors, 2021 Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim +4 more
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Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives
BioTechniques, 2012 Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms.
Yutaka Kodama, Chang-Deng Hu
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Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Viruses, 2021 The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts.
Aitor Nogales +5 more
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Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases
Frontiers in Molecular Biosciences, 2022 The recently discovered Rhodopsin-cyclases from Chytridiomycota fungi show completely unexpected properties for microbial rhodopsins. These photoreceptors function exclusively as heterodimers, with the two subunits that have very different retinal ...
Matthias Broser
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Frontiers in Chemistry, 2021 Single Molecule Localization Microscopy (SMLM) is an imaging method that allows for the visualization of structures smaller than the diffraction limit of light (~200 nm).
Pooja Laxman +3 more
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Fluorescent Probes for STED Optical Nanoscopy
Nanomaterials, 2021 Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong +2 more
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