Results 21 to 30 of about 1,328,987 (298)

Fluorescent Protein-Based Metal Biosensors

open access: yesChemosensors, 2023
Fluorescent proteins (FPs) are optical probes that are used to track the functions of genetically encoded target molecules in molecular and cellular biology.
Ki Hyun Nam
doaj   +1 more source

Improved split fluorescent proteins for endogenous protein labeling. [PDF]

open access: yes, 2017
Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact.
Feng, Siyu   +5 more
core   +2 more sources

Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)

open access: yesIndonesian Journal of Biotechnology, 2005
The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj   +1 more source

Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

open access: yesNature Communications, 2017
Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control ...
Cindy Ast   +6 more
doaj   +1 more source

Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]

open access: yes, 2008
The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Andrade, S.M.   +4 more
core   +2 more sources

The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles [PDF]

open access: yes, 2008
Copyright @ 2008 American Society for Microbiology.The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress.
Altmeyer, R   +11 more
core   +5 more sources

Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research

open access: yesScientific Reports, 2021
The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research.
Kara L. Schneider   +3 more
doaj   +1 more source

Optimizing the Protein Fluorescence Reporting System for Somatic Embryogenesis Regeneration Screening and Visual Labeling of Functional Genes in Cotton

open access: yesFrontiers in Plant Science, 2022
Protein fluorescence reporting systems are of crucial importance to in-depth life science research, providing systematic labeling tools for visualization of microscopic biological activities in vivo and revolutionizing basic research. Cotton somatic cell
Gai-Yuan Hu   +15 more
doaj   +1 more source

Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions

open access: yesFrontiers in Cellular and Infection Microbiology, 2017
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined ...
Kendal G. Cooper   +4 more
doaj   +1 more source

Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples. [PDF]

open access: yesPLoS ONE, 2007
BACKGROUND: Fluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of protein-protein interactions.
Joachim Goedhart   +4 more
doaj   +1 more source

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