Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy
Frontiers in Molecular Biosciences, 2021 We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.
Sangyoon Ko +4 more
doaj +1 more source
CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry. [PDF]
, 2008 Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization.
Balfe, Peter +12 more
core +2 more sources
Genetically Encoded Biosensors Based on Fluorescent Proteins
Sensors, 2021 Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim +4 more
doaj +1 more source
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
core +2 more sources
Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Viruses, 2021 The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts.
Aitor Nogales +5 more
doaj +1 more source
Surface modification of PDMS based microfluidic systems by tensides [PDF]
, 2013 The material aspects of a polymer based microfluidic structure were characterised considering the compatibility of the system with bioanalytical applications.
Fekete, Zoltán +2 more
core +1 more source
Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases
Frontiers in Molecular Biosciences, 2022 The recently discovered Rhodopsin-cyclases from Chytridiomycota fungi show completely unexpected properties for microbial rhodopsins. These photoreceptors function exclusively as heterodimers, with the two subunits that have very different retinal ...
Matthias Broser
doaj +1 more source
In Vivo Fluorescence Imaging of E-Selectin: Quantitative Detection of Endothelial Activation in Arthritis [PDF]
, 2010 Rheumatoid arthritis (RA) is a chronic progressive systemic inflammatory disease, characterized by synovial inflammation and localized destruction of cartilage and bone.
Gompels, Luke, Gompels, Luke
core +1 more source
Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives
BioTechniques, 2012 Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms.
Yutaka Kodama, Chang-Deng Hu
doaj +1 more source
On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters [PDF]
, 2013 Novel pathways of the synthesis of photoluminescent gold quantum clusters (AuQCs) using biomolecules as reactants provide biocompatible products for biological imaging techniques.
A Baksi +48 more
core +2 more sources