Results 31 to 40 of about 1,322,177 (348)
Frontiers in Plant Science, 2022 Protein fluorescence reporting systems are of crucial importance to in-depth life science research, providing systematic labeling tools for visualization of microscopic biological activities in vivo and revolutionizing basic research. Cotton somatic cell
Gai-Yuan Hu +15 more
doaj +1 more source
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
core +2 more sources
Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives
BioTechniques, 2012 Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a key technique to visualize protein-protein interactions in a variety of model organisms.
Yutaka Kodama, Chang-Deng Hu
doaj +1 more source
Scientific Reports, 2021 The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research.
Kara L. Schneider +3 more
doaj +1 more source
Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy
Frontiers in Molecular Biosciences, 2021 We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is a V2L mutant of UnaG with twice brighter bulk fluorescence.
Sangyoon Ko +4 more
doaj +1 more source
Genetically Encoded Biosensors Based on Fluorescent Proteins
Sensors, 2021 Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim +4 more
doaj +1 more source
Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Viruses, 2021 The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts.
Aitor Nogales +5 more
doaj +1 more source
Molecular Systems Biology, 2020 Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies.
Jiegen Wu +4 more
doaj +1 more source
Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases
Frontiers in Molecular Biosciences, 2022 The recently discovered Rhodopsin-cyclases from Chytridiomycota fungi show completely unexpected properties for microbial rhodopsins. These photoreceptors function exclusively as heterodimers, with the two subunits that have very different retinal ...
Matthias Broser
doaj +1 more source
Near-infrared fluorescent proteins [PDF]
Nature Methods, 2010 Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red ...
Dmitry, Shcherbo +14 more
openaire +2 more sources