Results 81 to 90 of about 1,322,177 (348)

Recent advances in the synthesis and application of fluorescent α-amino acids [PDF]

, 2016
Fluorescence spectroscopy has become a powerful technique for probing a range of complex biological processes including enzyme mechanisms and protein-protein interactions. While the application of this technique uses a number of strategies, many of these
Harkiss, Alexander, Sutherland, Andrew
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display

Frontiers in Plant Science, 2017
Plant viruses are suitable as building blocks for nanomaterials and nanoparticles because they are easy to modify and can be expressed and purified using plants or heterologous expression systems.
Juliane Röder, Rainer Fischer, Ulrich Commandeur   +2 more
doaj   +1 more source

Protein Mobility in the Cytoplasm of Escherichia coli [PDF]

, 1999
The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green
Elowitz, Michael B., Leibler, Stanislas, Stock, Jeffrey B., Surette, Michael G., Wolf, Pierre-Etienne   +4 more
core   +2 more sources

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore, Arthur, Atkinson, Bird, Brune, Byrd, Byrd, Chao, Cheng, Christopher P. Toseland, Corrie, Dillingham, Dillingham, Dillingham, Dillingham, Dillingham, Fili, Fili, Gorbalenya, Greenstein, Gregory I. Mashanov, Iordanescu, Justin E. Molloy, Kunzelmann, Leatherbarrow, Lee, Levin, Liisa T. Chisty, Lohman, Lohman, Manosas, Mark S. Dillingham, Martin R. Webb, Mashanov, Natalia Fili, Niedziela-Majka, Noirot, Sellers, Singleton, Slatter, Soultanas, Soultanas, Soultanas, Subramanya, Thomas, Thomas, Toseland, Veaute, Velankar, Webb, Yamaguchi, Yang, Yarranton, Yeeles, Zhang   +54 more
core   +1 more source

PICALM::MLLT10 translocated leukemia

FEBS Letters, EarlyView.
This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen, Antonia C. Nakatsugawa, Natalie Barton, Henry Haines, Gary S. Stein, Janet L. Stein, Daniel S. Wechsler, Jessica L. Heath   +7 more
wiley   +1 more source

Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. [PDF]

, 2012
Protein synthesis is highly regulated throughout nervous system development, plasticity and regeneration. However, tracking the distributions of specific new protein species has not been possible in living neurons or at the ultrastructural level ...
Butko, Margaret T, Ellisman, Mark H, Geng, Yang, Jeon, Noo Li, Kim, Hyung Joon, Lin, Michael Z, Mackey, Mason R, Shu, Xiaokun, Tsien, Roger Y, Yang, Jin   +9 more
core   +1 more source

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada, Barbero, Andrea, Candrian, Christian, Gianni-Barrera, Roberto, Jaquiery, Claude, Juelke, Henriette, Mainil-Varlet, Pierre, Martin, Ivan, Miot, Sylvie, Pelttari, Karoliina   +9 more
core   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source