Results 131 to 140 of about 17,088 (300)

Flow cytometry‐based monitoring of chimeric antigen receptor (CAR) T cells: Reagent selection, assay design, and clinical utility

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post‐infusion CART expansion and persistence and for real‐time clinical decision‐making. Multiparameter flow cytometry (MFC) enables rapid, live‐cell detection with absolute quantification and concurrent immunophenotypic characterization. This review
Jianhua Ling, Wei Wang, Sa A. Wang
wiley   +1 more source

Evaluation of a multiple myeloma synthetic cell mimic as an assay control for flow cytometry

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract The reliability of clinical flow cytometry assays hinges on the availability of robust assay controls that accurately reflect representative samples. Widely used conventional assay controls, such as cell lines, often fail to replicate the phenotypical and biophysical properties of representative primary samples resulting in gating variability ...
Dhong Hyun Lee, Mike Liu, Kanwal Palla, Subhanip Biswas, Swetha Pratyusha Gunturu, Brian Kerfs, Kristine Valenteros, Sarah Kotanchiyev, Shari Kaiser, Justine Dell'Aringa   +9 more
wiley   +1 more source

A practical difference from the normal approach to evaluate natural killer (NK) cells and NK cell neoplasms by flow cytometry

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Natural killer (NK) cell neoplasms span indolent to highly aggressive entities, and flow cytometric immunophenotyping is central to their detection and classification. However, distinguishing reactive from neoplastic NK cell expansions can be challenging because NK cells lack somatically recombined antigen receptors, and reactive states may ...
Aaron J. Wilk, Afshin Shameli, Erik Ames, Jonathan R. Fromm, Bartosz Grzywacz, Min Shi, Sindhu Cherian, Jean Oak   +7 more
wiley   +1 more source

Old data, new tricks: Comprehensive computational analysis of 10 years of multi‐center EuroFlow acute myeloid leukemia diagnostic immunophenotypic data

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Acute myeloid leukemia (AML) is characterized by high genotypic and immunophenotypic heterogeneity. We collected an extensive dataset containing 5366 flow cytometry files from 885 AML patients, stained with the EuroFlow acute leukemia orientation tube (ALOT) and AML/MDS panel, acquired in a standardized way at eight centers over a period of 10 
Sarah Bonte, Rosan Olsman, Sofie Van Gassen, Sergio Matarraz, Neus Villamor, Stefan Nierkens, Paula Fernandez, Elaine Sobral da Costa, Carmen‐Mariana Aanei, Alberto Orfao, Jacques J. M. van Dongen, Yvan Saeys, Mattias Hofmans, Vincent H. J. van der Velden   +13 more
wiley   +1 more source

Subset‐specific and tissue‐specific expression of NK‐cell markers and killer immunoglobulin‐like receptors: Implications for NK‐cell clonality assessment

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Accurate immunophenotypic evaluation of natural killer (NK)‐cell neoplasms is challenging because NK‐cells lack somatically rearranged antigen receptors, are comprised of phenotypically distinct subsets, and exhibit tissue‐specific subset distribution. We developed a flow cytometry (FC) panel for NK‐cell immunophenotypic analysis and clonality
Valerie Woodings, Orrin Koepke, Jason E. Love, Alireza Torabi, Lianqun Qiu, Jonathan R. Fromm, Sindhu Cherian, Afshin Shameli   +7 more
wiley   +1 more source

Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes

, 2020
Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA 3 e DAPI. The females have 2n = 34 chromosomes (2K = 32M+2A).
Lucio Antônio, Marthe, Graças Pompolo, Jefferson De Brito, Tânia Oliveira Campos, Maria Fernandes, Salomão, Mara Garcia Tavares, Silvia Das   +8 more
core  

Jaw‐specific stem cell dynamics are associated with distinct enamel architecture in mouse incisors

Developmental Dynamics, EarlyView.
Abstract Background The continuously growing mouse incisor serves as a model for stem cell‐driven tooth renewal. Although mice possess maxillary and mandibular incisors, most studies have focused on the mandible. Potential differences in structure and renewal between these serially homologous organs remain insufficiently characterized.
Elias Shahin, Jihan Asmar, Sanako Takano, Jimmy K. Hu, Amnon Sharir   +4 more
wiley   +1 more source

УЛЬТРАЗВУК МОЖЕТ ИЗМЕНЯТЬ ПРОНИЦАЕМОСТЬ ЗАРОДЫШЕВЫХ ОБОЛОЧЕК АМФИБИЙ

Ветеринарная патология, 2007
It was demonstrated that continuous ultrasound modifies the permeability of embryonic envelope of grass frog Rana temporaria for fluorochromes ANS, FDA and fluorescein.

doaj  

Composition of allogeneic equine orthobiologics and their repeated intra‐articular administrations

Equine Veterinary Journal, EarlyView.
Abstract Background Orthobiologics such as platelet‐rich plasma (PRP) and alpha‐2‐macroglobulin (A2MG) are increasingly used for equine musculoskeletal injuries. However, their composition and safety with repeated allogeneic intra‐articular administration remain poorly investigated.
Yanne Van Reusel, Sarah Y. Broeckx, Anna Carolo, Marco Patruno, Jonas Steenbrugge, Jimmy Saunders, Enrico Gugliandolo, Jan H. Spaas   +7 more
wiley   +1 more source

Metrnβ drives sepsis immunosuppression via macrophage reprogramming: A novel prognostic biomarker and therapeutic target

Interdisciplinary Medicine, EarlyView.
Metrnβ serves as a novel prognostic biomarker for sepsis and that targeted blockade of the Metrnβ‐c‐Kit axis represents a promising therapeutic strategy for sepsis. Abstract Sepsis is a heterogeneous syndrome critically driven by immunosuppression, yet lacking personalized prognostic markers and therapeutic targets. Here, we provide evidence to support
Xiao Li, Ai Chen, Chen Zhang, Meihong Da, Yonghui Liu, Zhi He, Chenyan Yuan, Shuo Ma, Yuming Yao, Dawen Chen, Meiling Zhou, Zhixin Song, Wenjing Lu, Chun‐Kwok Wong, Guoqiu Wu, Xun Gao   +15 more
wiley   +1 more source