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Physiological assessment of bacteria using fluorochromes

Journal of Microbiological Methods, 1995
This minireview focuses on the application of fluorogenic compounds in the detection of bacteria with particular emphasis on the assessment of physiological activity using epifluorescence microscopy. Microbiological applications of several related methods will also be reviewed.
G A, McFeters   +3 more
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Conjugation of Fluorochromes to Antibodies

2009
Immunolocalization of antigen via fluorescence requires that fluorochromes be linked either to the primary antibody (direct method) or to a second antibody (indirect method) to provide a fluorescent signal to mark the site of antibody-antigen binding. Of these two methods, the indirect technique is generally more useful and practical. Fluorochromes can
Su-Yau, Mao, J Michael, Mullins
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Optimizing multiple fluorochrome bone histodynamic markers

Bone, 1992
In vivo fluorochromes that adsorb to bone mineral, principally tetracyclines and their derivatives are well established histologic markers for assessing the mineralizing surfaces of bone (5). To distinguish clearly which surfaces are adsorbing fluorochrome at a particular time, fluorochromes of sufficiently different emission spectra to appear as ...
M, Mendes, K P, Pritzker
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Fluorochromes: Properties and Characteristics

2009
Immunofluorescence microscopy provides a sensitive means by which antigens can be localized within tissues or individual cells. For the most effective use of this technique the researcher can draw upon basic information on factors that affect the brightness of the fluorescence image, and how well that image can be distinguished from background ...
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Fluorochromes for DNA Staining and Quantitation

2017
In these last few decades the great explosion of the molecular approaches has casted a little shadow on the DNA quantitative analysis. Nevertheless DNA cytochemistry represented a long piece of history in cell biology since the advent of the Feulgen reaction.
Mazzini G, Danova M
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Stimuli-fluorochromic smart organic materials

Chemical Society Reviews
This review highlights the developments in stimuli-fluorochromic π-conjugated solids beyond organometallic and organic light-responsive compounds, addressing the design strategies, mechanisms, limitations, challenges, and opportunities.
Yinjuan Huang   +5 more
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Heterochromatin recognition with fluorochromes

Chromosoma, 1970
Some types of acridine derivative and especially Quinacrine dihydrochloride and its mustard may be successfully used as chromosome marker and to investigate the chemical differentiation of euchromatin and heterochromatin. — There are at least four main types of heterochromatin, showing all possible combinations of positive and negative cold effect ...
openaire   +1 more source

Polychrome labeling of bone with seven different fluorochromes: Enhancing fluorochrome discrimination by spectral image analysis

Bone, 2005
Bone formation and remodeling in vivo can be assessed by polychrome labeling using calcium-binding fluorescent dyes. The number of fluorochromes, however, limits this technique due to the fact that with increasing number, fluorescent spectra inevitably overlap, which makes discrimination more difficult.
Christoph, Pautke   +7 more
openaire   +2 more sources

Fluorochromes and Fluorescence

2007
To facilitate flow cytometry, there is a need for cells to be labeled with fluorochromes. However, because of the spectral properties of fluorochromes, the fluorescence associated with one fluorochrome may be detected by more than one detector, producing spectral overlap which requires compensation.
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Fluorescence and fluorochromes

2000
Abstract Fluorescence occurs when a molecule excited by light of one wavelength returns to the unexcited (ground) state by emitting light of a longer wavelength. The exciting and emitted light, being of different wavelengths, can be separated from one another using optical filters.
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