Results 41 to 50 of about 15,492 (288)

Spectral Cytometry Has Unique Properties Allowing Multicolor Analysis of Cell Suspensions Isolated from Solid Tissues. [PDF]

PLoS ONE, 2016
Flow cytometry, initially developed to analyze surface protein expression in hematopoietic cells, has increased in analytical complexity and is now widely used to identify cells from different tissues and organisms. As a consequence, data analysis became
Sandrine Schmutz, Mariana Valente, Ana Cumano, Sophie Novault   +3 more
doaj   +1 more source

Long‐Term Effects of Xenotransplantation of Human Enteric Glia in an Immunocompetent Rat Model of Acute Brain Injury

Advanced Science, EarlyView.
Acute brain injuries are characterized by extensive tissue damage, resulting in debilitating deficits in patients. Despite considerable progress, cell‐based approaches have yet to identify an ideal candidate. This long‐term study explores the use of an untested cell source – human enteric glia – and a non‐invasive administration route – intranasal ...
Nina Colitti, Edwige Rice, Franck Desmoulin, Maylis Combeau, Mélissa Parny, Lorenne Robert, Etienne Buscail, Barbara Bournet, Nathalie Vergnolle, Isabelle Raymond‐Letron, Isabelle Loubinoux, Carla Cirillo   +11 more
wiley   +1 more source

Imaging DNA with Fluorochrome Bearing Metals [PDF]

Inorganic Chemistry, 2013
Molecules that fluoresce upon binding DNA are widely used in assaying and visualizing DNA in cells and tissues. However, using light to visualize DNA in animals is limited by the attenuation of light transmission by biological tissues. Moreover, it is now clear that DNA is an important mediator of dead cell clearance, coagulation reactions, and an ...
Hoonsung, Cho, Yanyan, Guo, David E, Sosnovik, Lee, Josephson   +3 more
openaire   +2 more sources

FAPα+ Macrophages Orchestrate Immune Evasion in Multiple Myeloma by Dual Regulation of PD‐L1 and T Cell Senescence

Advanced Science, EarlyView.
ABSTRACT Multiple myeloma (MM) is a hematologic malignancy driven by clonal expansion of malignant plasma cells. Despite the long‐term disease control achieved with immunotherapies in some patients, treatment resistance remains a major cause of disease relapse.
Huiyao Gu, Zhenfeng Dai, Xi Huang, Enfan Zhang, Xinyuan Dai, Haoguang Chen, Wen Cao, Jinna Zhang, Yifan Hou, Haimeng Yan, Yang Yang, Li Yang, Yi Li, Wenlong Lin, Zhen Cai, Jingsong He   +15 more
wiley   +1 more source

Miniaturized Multichannel Near Infrared Endoscope for Mouse Imaging

Molecular Imaging, 2003
We describe the design and construction of a miniaturized multichannel near infrared (NIR) endoscopic imaging system developed for high-resolution imaging of mice.
Martin A. Funovics, Herlen Alencar, Henry S. Su, Khashayarsha Khazaie, Ralph Weissleder, Umar Mahmood   +5 more
doaj   +1 more source

Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

Molecules, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, László G. Puskás, Gabriella Fábián, Liliána Z. Fehér, Pradeep Kumar Reddy Cingaram, Bhavyashree Suresh, Begüm Peksel, Soujanya Kuntam, Eszter Molnár   +10 more
doaj   +1 more source

Elemene Augments the Effects of Anti‐PD‐1 Immunotherapy on Hepatocellular Carcinoma by Regulating the miR‐130a‐5p/SPP/MHC‐I Axis

Advanced Science, EarlyView.
Elemene increases SPP expression by competitively binding with miR‐130a‐5p to suppress SPP mRNA degradation. This led to more antigen/MHC‐I complexes being expressed on the cell surface, which consequently facilitated the recognition and killing of HCC cells by CTLs and enhancing the antitumor immune efficacy of anti‐PD‐1.
Menglan Wang, Mengqing Sun, Heng Dong, Xiaoting Zhang, Jingbo Zhang, Zhengguo Zhang, Mengjie Ni, Lina Li, Yunxin Pei, Xiaoyu Chen, Qian Li, Fangtian Bu, Jiacheng Huang, Liangyu Jiang, Zhuting Fang, Xuliang Chen, Jianxiang Chen, Yiting Qiao, Tian Xie   +18 more
wiley   +1 more source

Flow Cytometry for Estimating Plant Genome Size: Revisiting Assumptions, Sources of Variation, Reference Standards, and Best Practices

Journal of the American Society for Horticultural Science
Flow cytometry has been widely used to estimate relative and absolute genome sizes (DNA contents) of plants for more than 50 years. However, the accuracy of these estimates can vary widely because of many factors, including errors in the genome size ...
John Nix, Thomas G. Ranney, Nathan P. Lynch, Hsuan Chen   +3 more
doaj   +1 more source

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

Genetics and Molecular Biology, 2004
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization
Monique Mantovani, Luciano Douglas dos Santos Abel, Carlos Alberto Mestriner, Orlando Moreira-Filho   +3 more
doaj   +1 more source

Nanoparticle Adjuvant Design Enhances Germinal Center Responses Targeting Conserved Subdominant Epitopes for Pan‐Coronavirus Vaccine Development

Advanced Science, EarlyView.
Current SARS‐CoV‐2 vaccines generate short‐lived, strain‐specific immunity. A polymeric TLR7 agonist nanoparticle (TLR7‐NP) adjuvant enhances lymph node targeting and promotes early and sustained germinal center responses toward conserved S2 epitopes of the spike protein.
Sijin Huang, Kanella M. Cohen, Liqiang Chen, Xiaowo Kang, Chang Liu, Megan E. Demouth, Wenxia Jiang, Alexander R. Maldeney, Rong Tong, Zunlong Ke, Kartik Chandran, Wei Luo, Qian Yin   +12 more
wiley   +1 more source