Results 51 to 60 of about 17,088 (300)

Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

STAR Protocols, 2021
Summary: This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in
Emma K. Jennings, David A.J. Lecky, Masahiro Ono, David Bending   +3 more
doaj   +1 more source

Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments [PDF]

, 1982
Unsynchronized cells of an essentially diploid strain of female Chinese hamster cells derived from lung tissue (CHL) were laser-UV-microirradiated (=257 nm) in the nucleus either at its central part or at its periphery.
Cremer, Thomas, Schneider, T., Schneider, Tilman, Baumann, Hella, Kirsch-Volders, Micheline, Cremer, Christoph, Hens, Luc, Cremer, Thomas John T., Kirsch-Volders, M.   +8 more
core   +1 more source

Engineered Xenogeneic Bone Scaffold with IL‐10 Nanodelivery System: Immunomodulation and BMSC Fate Programming for Skull Defect Repair

Advanced Science, EarlyView.
A multifunctional regenerative composite is constructed by 3D‐printing thermosensitive PNIPAM hydrogel embedded with MPDA@IL‐10 nanoparticles and autologous BMSCs onto antigen‐depleted porcine bone matrix for beagle critical‐sized skull defect reconstruction.
Weihao Lv, Lei Zhang, Fei Fei, Zhibin Wu, Xiuquan Wu, Ya‐nan Dou, Yihao Fu, Hao Chang, Yujie Qiang, Huaxia Xie, Ni An, Leiying Chen, Hongchen Zhang, Min‐Wen Zheng, Hongqing Chen, Xia Li, Zhou Fei   +16 more
wiley   +1 more source

Fluorescent markers in microscopy: photophysical characteristics and applications in cell biology

Acta Biologica Slovenica, 2016
In the fluorescence microscopy of biological specimens the structures in cells and tissues usually need to be labelled with various fluorescent markers.
Urban Bogataj, Jasna Štrus, Nada Žnidaršič, Marko Kreft   +3 more
doaj   +1 more source

Spectral Cytometry Has Unique Properties Allowing Multicolor Analysis of Cell Suspensions Isolated from Solid Tissues. [PDF]

PLoS ONE, 2016
Flow cytometry, initially developed to analyze surface protein expression in hematopoietic cells, has increased in analytical complexity and is now widely used to identify cells from different tissues and organisms. As a consequence, data analysis became
Sandrine Schmutz, Mariana Valente, Ana Cumano, Sophie Novault   +3 more
doaj   +1 more source

Repurposing a Small Molecule Plant Hormone as a Tunable ON‐Switch for CAR‐T Cell Immunotherapy

Advanced Science, EarlyView.
By engineering a receptor system integrating the plant auxin receptor AFB1 with its co‐receptor IAA7, we enable ligand‐dependent interactions triggered by the plant hormone auxins. This design allows rapid, reversible, and dose‐dependent T cell activation, resulting in potent cytotoxicity against B‐cell lymphoma in vitro and in vivo.
Hongxiang Zeng, Lei Zheng, Rongkun Tao, Sirui Zheng, Yanke Zhong, Yongxia Niu, Peng Zhang, Xiang Ma, Chen Yang, Min Zhang, Fuyu Duan, Jing Yang, Xuan Wei, Xiang Zhou, Zhenbiao Yang, Zhaoxi Sun, Qizhou Lian   +16 more
wiley   +1 more source

APPLICATION OF FLOW CYTOMETRY TO ASSESS THE ABUNDANCE AND PHYSIO LOGICAL STATE BACTERIA OF HIGH BOG PEAT

Siberian Journal of Life Sciences and Agriculture
Background. Peat from upland bogs, as a substrate, is characterized by a number of specific conditions that make it difficult to separate microbial cells for cytometric analysis.
Dmitriy A. Shpanov, Ivan N. Zubov
doaj   +1 more source

circZNF148 Drives Glucose Metabolism Reprogramming to Enhance Metastasis and Immune Evasion via HK1 Stabilization in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
CircZNF148 stabilizes HK1 through deubiquitinase recruitment, thereby enhancing glycolysis and lactate production. Elevated lactate promotes PD‑L1 lactylation and membrane accumulation while suppressing CD8+ T‐cell cytotoxicity, collectively facilitating immune evasion and malignant progression in TNBC.
Yuhan Jin, Wenjing Zhao, Lei Wang, Jianing Wang, Keying Zhao, Dan Luo, Yifei Wang, Yuxia Yang, Jingze Yang, Yaming Li, Tong Chen, Dianwen Han, Zekun Wang, Xiaoyan Li, Bing Chen, Lijuan Wang, Yiran Liang, Qifeng Yang   +17 more
wiley   +1 more source

Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

Molecules, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, László G. Puskás, Gabriella Fábián, Liliána Z. Fehér, Pradeep Kumar Reddy Cingaram, Bhavyashree Suresh, Begüm Peksel, Soujanya Kuntam, Eszter Molnár   +10 more
doaj   +1 more source

Inorganic Nanomaterials can Promote or Inhibit Tumor Metastasis Depending on Tumor Microenvironment Composition

Advanced Science, EarlyView.
As engineered nanomaterials gain prominence in cancer imaging and therapy, understanding their impact on tumor progression becomes increasingly critical. We demonstrate that gold and silica‐coated gold nanomaterials can either suppress or enhance breast cancer metastasis through distinct tumor microenvironment interactions, while identifying ...
Kiana Buttiens, Xiaoqi Zheng, Xueqi Fan, Mukaddes Izci, Christy Maksoudian, Irati Perez Gilabert, Tianjiao Chu, Filipa Goncalves, Jonas Dehairs, Johan Swinnen, Bella B. Manshian, Stefaan J. Soenen   +11 more
wiley   +1 more source