Results 141 to 150 of about 222,091 (247)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Many Ways Out: Beyond the Two-State Model of Protein Unfolding. [PDF]

ACS Omega
Pastore A, Temussi PA.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Determinants of Protein Folding Pathways: Lessons from Metamorphic Proteins. [PDF]

Int J Mol Sci
Pennacchietti V, Di Felice M, Toso J, Caldarelli L, Ventura ES, Malagrinò F, Toto A, Gianni S.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Folding the message: mRNA structure as a regulatory layer of human mitochondrial gene expression. [PDF]

Biochim Biophys Acta Mol Cell Res
Ahn A, Hong S, Brischigliaro M, Fontanesi F, Barrientos A.   +4 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Structure-Based Simulations for Folding of a tRNA Isodecoder. [PDF]

Molecules
Levintov L, Orellana EA, Vashisth H.
europepmc   +1 more source

Cumulative Cosmic Folding Model: A Testable Framework

This version 2.1 of the Cumulative Cosmic Folding Model (CCFM) presents a testable modification to Penrose's Conformal Cyclic Cosmology. We propose that topological information is preserved across aeons, leading to the falsifiable prediction ∆α/α = β ln(n + 1) with β ~ 10⁻⁵. The model is testable with JWST, ELT-Andes, and CMB-S4 by 2030.
openaire   +2 more sources