Results 211 to 220 of about 542,089 (343)

Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803

open access: yesBiotechnology Progress, EarlyView.
Abstract D‐Mannitol production was achieved in freshwater Synechocystis sp. PCC6803 via the heterologous expression of mannitol‐1‐phosphate dehydrogenase (mtlD) and mannitol‐1‐phosphatase (m1p) under control of the strong promoter Ptrc1. However, only 5.54 mg L−1 of mannitol was found extracellularly after 7 days of cultivation, likely due to ...
Wenyang Wu   +3 more
wiley   +1 more source

Editorial: Emerging Frontiers in the Formation of Viable but Non-culturable Microorganisms and Biofilms During Food Processing

open access: yesFrontiers in Microbiology, 2021
Zhenbo Xu   +5 more
doaj   +1 more source

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

open access: yesBiotechnology Progress, EarlyView.
Abstract One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N‐1 stage) to seed the production culture (N stage) at ultra‐high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves cell culture performance by shortening the timeline and/or achieving higher final
Lucas Lemire   +5 more
wiley   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

open access: yesBiotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold   +11 more
wiley   +1 more source

A novel multiparameter sensor for shake flask cultivations: Online biomass, dissolved oxygen, and fluorescence monitoring for comprehensive bioprocess characterization

open access: yesBiotechnology Progress, EarlyView.
Abstract Shake flasks are one of the most widely used cultivation vessels in biotechnological process development. To improve the process understanding, new technologies have been reported for online monitoring of different parameters like oxygen, pH, or biomass in the last couple of years.
Lara Strehl   +4 more
wiley   +1 more source

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