Results 61 to 70 of about 110,269 (254)

The Establishment of Surface Roughness as Failure Criterion of Al–Li Alloy Stretch-Forming Process

open access: yesMetals, 2016
Taking Al–Li–S4–T8 Al–Li alloy as the study object, based on the stretching and deforming characteristics of sheet metals, this paper proposes a new approach of critical orange peel state characterizations on the basis of the precise measurement of ...
Jing-Wen Feng, Li-Hua Zhan, Ying-Ge Yang
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Determination of the forming limit diagram of zinc electro-galvanized steel sheets

open access: yesMetalurgija, 2012
Forming limit curves (FLC) of deep drawing steel sheets have been determined experimentally and calculated on the base of the material tensile properties following the Hill, Swift, Marciniak-Kuczyński and Sing-Rao methods.
W. Fracz, F. Stachowicz
doaj  

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

open access: yesFEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa   +2 more
wiley   +1 more source

Design and analysis strategies for robust microbiome ageing research

open access: yesFEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik   +5 more
wiley   +1 more source

Experimental Investigation of Forming Limit Curve for AA5754-O

open access: yesIOP Conference Series: Materials Science and Engineering, 2018
The Forming Limit Curve (FLC) is a line that is consisted of the major and minor strain pairs for different kinds of strain paths. It is widely adopted for the quantitative description of the sheet metal formability. In this paper, a stand-alone servo-motor apparatus associated with the high-accuracy measurement system was developed, based on which the
W N Yuan, M Wan, X D Wu
openaire   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

open access: yesFEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt   +8 more
wiley   +1 more source

A biaxial test for rheological and formability identification

open access: yesEPJ Web of Conferences, 2010
Accurate constitutive laws and formability limits of materials are essential for a numerical optimization of sheet forming processes. The main objective of this work is to develop a new experimental device able to give for a unique specimen a good ...
Ragneau E.   +3 more
doaj   +1 more source

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