Results 141 to 150 of about 1,361,829 (303)

New approaches on fractional integral inequalities for functions whose higher-order derivatives are bounded

Demonstratio Mathematica
In the present paper, utilizing a wide class of fractional integral operators (namely the Riemann-Liouville fractional integral operator) and some functions whose higher-order derivatives are absolutely continuous, we develop novel fractional integral ...
Erden Samet, Ozdemir Burçin Gokkurt, Yildiz Çetin, Cotîrlă Luminiţa-Ioana, Breaz Daniel   +4 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Fractional integral-like processing in retinal cones reduces noise and improves adaptation. [PDF]

PLoS One, 2018
Martinecz A, Niitsuma M.
europepmc   +1 more source

Fractional Brownian Motion as a Differentiable Generalized Gaussian Process [PDF]

Brownian motion can be characterized as a generalized random process and, as such, has a generalized derivative whose covariance functional is the delta function. In a similar fashion, fractional Brownian motion can be interpreted as a generalized random
Peter C.B. Phillips, Victoria Zinde-Walsh   +1 more
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hermite–Hadamard–Mercer type inequalities for fractional integrals: A study with h-convexity and ψ-Hilfer operators

Boundary Value Problems
In this paper, we first prove a generalized fractional version of Hermite-Hadamard-Mercer type inequalities using h-convex functions by means of ψ-Hilfer fractional integral operators.
Noureddine Azzouz, Bouharket Benaissa, Hüseyin Budak, İzzettin Demir   +3 more
doaj   +1 more source

Positive solutions of fractional integral equations by the technique of measure of noncompactness. [PDF]

J Inequal Appl, 2017
Nashine HK, Arab R, Agarwal RP, De la Sen M.   +3 more
europepmc   +1 more source