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Switching Frontal Polymerization Mechanisms: FROMP and FRaP
ACS Macro Letters, 2022Two frontal polymerization (FP) mechanisms, frontal ring-opening metathesis polymerization (FROMP) of dicyclopentadiene and frontal radical polymerization (FRaP) of benzyl acrylate and hexanediol diacrylate, were combined for rapid manufacturing of welded thermoset materials.
Jacob J. Lessard +5 more
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Long-Term Fluorescence Recovery After Photobleaching (FRAP)
2023Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers ...
Takumi, Saito +2 more
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Analysis of receptor oligomerization by FRAP microscopy
Nature Methods, 2009Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP.
Dorsch, Sandra +4 more
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FRAP analysis of binding: proper and fitting
Trends in Cell Biology, 2005Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of these interactions are frequently done using fluorescence recovery after photobleaching (FRAP). Proper interpretation of FRAP data yields information about the binding interactions of fluorescently tagged molecules, including the number of binding states and ...
Brian L, Sprague, James G, McNally
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Once a physicist: Thomas Fraps
Physics World, 2006My interest in science was first sparked by my grandfather, who was an engineer and mathematician. Later I started reading science-fiction and popular-science books by the likes of Carl Sagan and Douglas Hofstadter. I finally decided to study physics when I was doing my military service, which would have been an intellectual vacuum for me had it not ...
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Expanding the scope of quantitative FRAP analysis
Journal of Theoretical Biology, 2010In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in
Mark A, Hallen, Anita T, Layton
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Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies
Current Opinion in Biotechnology, 2005Protein mobility within cells is of key importance for many cellular functions. Although immunostaining can reveal protein locations in the steady-state, this might not represent the full picture and provides no information about protein movements. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) are two ...
Mario, Köster +2 more
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Auswertung von FRAP-Experimenten
2015Die Auswertung von FRAP-Experimenten nach AXELROD et al. [3] wird noch heute haufig verwendet, da sie relativ leicht durchzufuhren ist. Im Folgenden werden die Grundzuge der theoretischen Beschreibung eines FRAP-Experiments nach AXELROD et al. dargestellt, dabei wird nur auf seine Uberlegungen zur lateralen Diffusion in planaren Lipidmembranen ...
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Simulation von FRAP-Experimenten
2015Um die zuvor theoretisch beschriebenen Methoden einem Test zu unterziehen, sollen in diesem Kapitel Simulationen von FRAP-Experimenten durchgefuhrt werden. Die Simulation der Experimente wird mit der Programmiersprache PYTHON durchgefuhrt, speziell werden die PYTHON-Bibliotheken SCIPY[20] und NUMPY[20] fur numerische Berechnungen, Integrationen ...
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Molecular diffusion and binding analyzed with FRAP
Protoplasma, 2014Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced.
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