Results 21 to 30 of about 190,999 (265)

Operational experience with Adaptive Gain Integrating Pixel Detectors at European XFEL

Frontiers in Physics
The European X-ray Free Electron Laser (European XFEL) is a cutting-edge user facility that generates per second up to 27,000 ultra-short, spatially coherent X-ray pulses within an energy range of 0.26 to more than 20 keV.
Jolanta Sztuk-Dambietz, Vratko Rovensky, Alexander Klujev, Torsten Laurus, Ulrich Trunk, Karim Ahmed, Olivier Meyer, Johannes Möller, Andrea Parenti, Natascha Raab, Roman Shayduk, Marcin Sikorski, Gabriele Ansaldi, Ulrike Bösenberg, Lopez M. Luis, Astrid Muenich, Thomas R. Preston, Philipp Schmidt, Stephan Stern, Richard Bean, Anders Madsen, Luca Gelisio, Steffen Hauf, Patrick Gessler, Krzysztof Wrona, Heinz Graafsma, Heinz Graafsma, Monica Turcato   +27 more
doaj   +1 more source

An Optimized Approach for Serial Crystallography Using Chips

Crystals, 2023
Serial crystallography is a rapidly developing method for the determination of the structure of biomolecules at room temperature at near-atomic resolution from an ensemble of small crystals. Numerous advances in detectors, data analysis pipelines, sample
Marina Galchenkova, Aida Rahmani Mashhour, Patrick Y. A. Reinke, Sebastian Günther, Jan Meyer, Henry N. Chapman, Oleksandr M. Yefanov   +6 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Room-temperature X-ray fragment screening with serial crystallography

Nature Communications
Structural insights into protein-ligand interactions are essential for advancing drug development, with macromolecular X-ray crystallography being a cornerstone technique.
Sebastian Günther, Pontus Fischer, Marina Galchenkova, Sven Falke, Patrick Y. A. Reinke, Sreevidya Thekku Veedu, Ana Carolina Rodrigues, Johanna Senst, Daniel Elinjikkal, Lars Gumprecht, Jan Meyer, Henry N. Chapman, Miriam Barthelmess, Alke Meents   +13 more
doaj   +1 more source

MHz X-ray photon correlation spectroscopy using an acoustic levitator at the European XFEL

Journal of Synchrotron Radiation
The structural and dynamical properties of soft-matter systems play an important role in crystallization and nucleation theory. Despite their significance, the dynamical properties are still poorly understood because of experimental constraints and the ...
Wonhyuk Jo, Johannes Möller, Jörg Hallmann, James Wrigley, Jan-Etienne Pudell, Ulrike Boesenberg, Felix Brausse, Angel Rodriguez-Fernandez, Alexey Zozulya, Roman Shayduk, Anders Madsen   +10 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Efficient electronic structure calculation for molecular ionization dynamics at high x-ray intensity

Structural Dynamics, 2015
We present the implementation of an electronic-structure approach dedicated to ionization dynamics of molecules interacting with x-ray free-electron laser (XFEL) pulses.
Yajiang Hao, Ludger Inhester, Kota Hanasaki, Sang-Kil Son, Robin Santra   +4 more
doaj   +1 more source

The Quantum Free-Electron Laser [PDF]

, 2018
CERN Yellow Reports: School Proceedings, Vol 1 (2018): Proceedings of the CAS-CERN Accelerator School on Free Electron Lasers and Energy Recovery ...
openaire   +3 more sources

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source