Results 21 to 30 of about 190,999 (266)
Ultrashort ultraviolet free-electron lasers [PDF]
Journal of X-Ray Science and Technology, 1994 In this work we combine elements of chirped pulse amplification (CPA) techniques, now familiar in solid-state lasers, with an amplifier based upon a seeded free-electron laser (FEL). The resulting device would produce amplified pulses of unprecedented brevity at wavelengths shorter than can be currently obtained by any tunable laser system.
Umstadter, Donald +3 more
openaire +3 more sources
Bayesian Optimization of a Free-Electron Laser
Physical Review Letters, 2020 6 pages, 3 ...
J. Duris +10 more
openaire +4 more sources
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Room-temperature X-ray fragment screening with serial crystallography
Nature CommunicationsStructural insights into protein-ligand interactions are essential for advancing drug development, with macromolecular X-ray crystallography being a cornerstone technique.
Sebastian Günther +13 more
doaj +1 more source
MHz X-ray photon correlation spectroscopy using an acoustic levitator at the European XFEL
Journal of Synchrotron RadiationThe structural and dynamical properties of soft-matter systems play an important role in crystallization and nucleation theory. Despite their significance, the dynamical properties are still poorly understood because of experimental constraints and the ...
Wonhyuk Jo +10 more
doaj +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Efficient electronic structure calculation for molecular ionization dynamics at high x-ray intensity
Structural Dynamics, 2015 We present the implementation of an electronic-structure approach dedicated to ionization dynamics of molecules interacting with x-ray free-electron laser (XFEL) pulses.
Yajiang Hao +4 more
doaj +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Spectral phase control of interfering chirped pulses for high-energy narrowband terahertz generation
Nature Communications, 2019 Optical generation of terahertz radiation is needed for many applications, but gaining high efficiency is still a challenge. The authors report a method to overcome dispersion effects in interfering chirp pulses used for THz pulse production by tuning ...
Spencer W. Jolly +9 more
doaj +1 more source