Results 141 to 150 of about 2,106,991 (262)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Simulation of direct current converters using Python to improve electronics learning
Revista Científica de Ingeniería EnergéticaThe objective of the article is to propose the educational application on DC – DC converters based on the free software Python, which allows the analysis, experimentation, demonstration and design of the functions of DC – DC converters. Among the results
Josnier Ramos Guardarrama +3 more
doaj
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Reliability and validity of free software for the analysis of locomotor activity in mice. [PDF]
Yeungnam Univ J Med, 2018 Hong YR, Moon E.
europepmc +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis. [PDF]
SLAS Discov, 2019 Lee PH, Huang XX, Teh BT, Ng LM.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining. [PDF]
Front Neurosci, 2019 Shi XF, Ai H, Lu W, Cai F.
europepmc +1 more source
FEBS Open Bio, EarlyView.This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia +3 more
wiley +1 more source
HRVanalysis: A Free Software for Analyzing Cardiac Autonomic Activity. [PDF]
Front Physiol, 2016 Pichot V +4 more
europepmc +1 more source