Results 81 to 90 of about 2,038,816 (256)

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Analysis of Harbin’s Freeze-Thaw Action for Life-Cycle Structure Design

, 2011
To get environmental action information for structural life cycle design and durability design, the air temperature of Harbin (China) from 1963 to 2002 was employed to analyze the probability distribution of freeze-thaw action herein.
Chi Lin, Jin Ping Ou
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Durability of E-Glass Fiber Reinforced Polymer Subjected to Freeze-Thaw Cycle and Sustained Load

, 2010
Strengthening concrete structure with E-glass fiber reinforced polymer (GFRP) is gaining more popularity, mainly due to their superior mechanical properties. However, there is insufficient information on environmental durability of glass fiber reinforced
Yi Yan Lu, Shan Li, Hui Tao Ren
core   +1 more source

Research on damage and degradation of coal-bearing sandstone under freeze-thaw cycles

Frontiers in Earth Science
Comprehending the effect of freeze-thaw cycles on the damage and degradation of coal-bearing sandstones is crucial for the end-wall slope stability of open-pit mines in cold areas.
Yiwen Mao, Ming Li, Peng Wu, Shuai Guo, Fuqiang Zhu   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Disparity in soil erosion processes between freeze-thaw and unfrozen slopes under artificial rainfall conditions in high-altitude and dry valleys of the Southeast Tibet region

International Soil and Water Conservation Research
The arid valley region in southeastern Tibet exemplifies an ecologically vulnerable area in southwestern China, where soil erosion has intensified in recent years as a consequence of socio-economic growth and infrastructure construction. This study aimed
Yao Xiao, Qunou Jiang, Weihang Zhao, Zuoxiao Wang, Rui Xiong, Jing Li, Linjuan He   +6 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Changing of mechanical property and bearing capacity of strongly chlorine saline soil under freeze-thaw cycles

Scientific Reports
Freeze-thaw cycles and compactness are two critical factors that significantly affect the engineering properties and safety of building foundations, especially in seasonally frozen regions. This paper investigated the effects of freeze-thaw cycles on the
Shijun Ding, Shaomin Li, Sen Kong, Qiuyang Li, Taohui Yang, Zhibao Nie, Gaowen Zhao   +6 more
doaj   +1 more source