Results 221 to 230 of about 156,848 (283)

AlN-based aerogel thermo-cooler enabled by enhanced phonon conduction and unconstrained liquid capillarity. [PDF]

Nat Commun
Meng S, Wang H, Zhao Q, Lü X, Yu J, Si Y.   +5 more
europepmc   +1 more source

Mathematical model of permeability evolution of liquid CO₂ pressurized coal. [PDF]

Sci Rep
Li C, Cheng Q, Bai G, Zhou X, Zhang F, Zhang W.   +5 more
europepmc   +1 more source

Mechanical properties and microstructural analysis of MICP-reinforced coarse-grained saline soils under freeze-thaw cycling. [PDF]

PLoS One
Xiong L, Tian L, Zhang X, Wang M, Ahemaiti A.   +4 more
europepmc   +1 more source

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Freeze-fracture-autoradiography

Histochemistry, 1976
A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing.
E, Rix, A, Schiller, R, Taugner
openaire   +2 more sources

Complementary freeze‐fracture, freeze‐etch specimens

Journal of Microscopy, 1979
SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
openaire   +2 more sources

Freeze-fracture immunogold labeling

Histochemistry and Cell Biology, 1996
Several approaches have been developed to combine immunogold cytochemistry and freeze-fracture techniques. These methods are highly heterogeneous regarding both the sequence of the procedural steps and the aspect of the resulting images. They imply immunolabeling either before or after freeze-fracture or even immunolabeling of platinum/carbon replicas ...
TORRISI, Maria Rosaria, MANCINI, Patrizia   +1 more
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Freeze Fracture and Freeze Etching

2013
Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture.
Douglas E, Chandler, William P, Sharp
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Freeze-Fracture Enzyme Cytochemistry: Application of Enzyme Cytochemistry to Freeze-Fracture Cytochemistry

Journal of Electron Microscopy, 1996
We present a novel freeze-fracture cytochemistry method based upon enzyme cytochemistry. By this method, freeze-fractured membranes are labeled with cerium as an enzyme cytochemical marker on replicas. The cerium capture method was suitable for freeze-fracture enzyme cytochemistry because the cerium phosphate reaction product is stable after replica ...
T, Takizawa, T, Saito
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A simple and reliable quick-freezing/freeze-fracturing procedure

Histochemistry and Cell Biology, 1997
We describe a simple method for the quick-freezing/freeze-fracturing of cells in tissues or culture monolayers. Tissue slices or cultured cells were covered with thin copper foil (10-micron-thick), and frozen by smashing them against a liquid helium-cooled copper block.
K, Fujimoto, T, Noda, T, Fujimoto
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Combined Freeze-Fracturing and Autoradiography Techniques: Freeze-Fracture Autoradiography

1981
In the past many efforts have been made to apply classical techniques of fixation and embedding to the autoradiography of soluble material. There is general agreement today that this goal can only be reached by cryotechniques (Amlacher 1974).
A. Schiller, R. Taugner
openaire   +1 more source