Results 231 to 240 of about 156,848 (283)

Freeze‐fracture of manufactured foods

Journal of Electron Microscopy Technique, 1989
AbstractManufacture of food products with desired texture and stability increasingly benefits from an understanding of the food microstructure. Freeze‐fracture is advantageous in gaining this understanding because it avoids alterations of hydration, retains fat, and maximally preserves air cells.
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Freeze-Etching and Freeze-Fracturing

1973
The freeze-etching (or fracturing) technique involves the making of a platinum-carbon replica of the fracture face through frozen cells. This replica is examined in the electron microscope. There are two advantages compared with earlier techniques. Firstly, fixation and dehydration are not necessary and the replica is thus of cells which differ from ...
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Freeze‐fracture: A personal history

Journal of Electron Microscopy Technique, 1989
AbstractThe sequence of events leading to the development of freeze‐fracture replication is described. Subsequent developments discussed include complementary replicas, replica interpretation with stereo micrograph and reversal negatives, replica reinforcement, and control of resistance evaporation.
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Freeze-fracture electron microscopy

Nature Protocols, 2007
The freeze-fracture technique consists of physically breaking apart (fracturing) a frozen biological sample; structural detail exposed by the fracture plane is then visualized by vacuum-deposition of platinum-carbon to make a replica for examination in the transmission electron microscope.
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Complementary freeze-fracture and freeze-etch preparations

Proceedings, annual meeting, Electron Microscopy Society of America, 1984
Whereas freeze-etching technology was introduced in 1957 (1) and the first freeze-etching equipment to be made available commercially was developed in 1961 (2), the need for complementary replicas was not recognized until Branton (3) proposed that the two membrane faces seen in freeze-etched specimens were not the inner and outer surfaces of membranes ...
Russell L. Steere, Eric F. Erbe, Bryan K. Bartle   +2 more
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Freeze‐fracturing and freeze‐etching of cardiac myosin filaments

Journal of Microscopy, 1975
SUMMARYMyofilament structure was studied in freeze‐etch replicas of unfixed, glycerinated beef cardiac muscle. The information which is revealed depends upon the direction of metal shadowing in relation to the filament axis. Shadows oblique to this axis reveal that the outer surface of a longitudinal half of a thick filament comprises three, sometimes ...
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Freeze-fracture of intact sarcotubular membranes

Journal of Ultrastructure Research, 1978
Pectoralis muscle from normal and dystrophic chickens was examined following preparation by the technique of freeze-fracturing. The intramembranous particle density of both the cytoplasmic and extracellular (luminal) faces of the junctional sarcoplasmic reticulum (SR), free SR, and T system of normal and dystrophic chickens was determined and subjected
L M, Crowe, R J, Baskin
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Freeze Fracture of Intact Plant Tissues

Stain Technology, 1982
A procedure for dissolving and handling replicas of cutinized leaves and other plant tissues is described. This technique yields comparatively large replicas which can include vascular tissue, epidermal cells and the cuticle. Dissolution of the tissue involves the sequential use of alcoholic KOH, sulfuric acid and chromic acid.
K A, Platt-Aloia, W W, Thomson
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Hodgkin cells in freeze-fracture replicas

Virchows Archiv B Cell Pathology, 1988
Lymph nodes from six patients with Hodgkin's disease (three with the nodular sclerosing subtype, one with mixed cellularity and two with the lymphocyte-predominant subtype) were analysed by electron microscopy in freeze-fracture replicas and thin sections.
E, Kaiserling, H, Wolburg, K, Berg
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A Technique for Freeze-Fracturing of the Organ of Corti

Acta Oto-Laryngologica, 1985
A modification of the conventional method of freeze-fracturing is described. By the use of polyvinyl-alcohol, a high viscous fluid, the organ of Corti is better stabilized in the specimen holder. This fluid also facilitates a clean fracture through the organ of Corti proper and in a high number of cases gives a large replica void of contaminating ...
B, Engström, M, Hoffstedt, D, Bagger-Sjöbäck   +2 more
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