Results 241 to 250 of about 156,848 (283)

Freeze-fracture of adrenal chromaffin granules

Neuroscience, 1977
Abstract Freeze-fracture replicas of bovine adrenal chromaffin granules show convex and concave fracture faces. The concave fracture faces are studded with 9 nm diameter particles (on average 950/μm 2 ) while the convex fracture faces are almost devoid of particles (approx 150/μm 2 ).
P A, Eagles, L N, Johnson, C, Van Horn, S, Bullivant   +3 more
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Freeze‐fracture for scanning electron microscopy

Journal of Microscopy, 1977
SUMMARYTwo different freeze‐fracture methods are explored for preparation of biological material for scanning electron microscopy. In the simpler method the tissues are first fixed and dehydrated. They are then frozen and fractured, and after thawing, critical‐point dried.
G H, Haggis, B, Phipps-Todd
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Freeze-fracture studies on lipids and membranes

Micron, 2001
Freeze-fracture electron microscopy is especially useful for investigation of lipid structures by the advantageous fracture course within hydrophobic zones. Freezing is, on the other hand, a restriction because the structures of lamellar and non-lamellar phase states with disordered acyl chains (L(alpha), H(II,) cubic) are difficult to preserve.
H W, Meyer, W, Richter
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Observations on freeze-fractured membranes of a trypanosome

Tissue and Cell, 1974
Abstract Pure preparations of Trypanosoma brucei , free from plasma and cellular components were isolated from rat blood, and concentrated into loose pellets by low-speed centrifugation. Pellets were either processed for thin sectioning as a control for general morphology, or glycerol-treated after glutaraldehyde fixation for preparation of freeze ...
D S, Smith, A R, Njogu, M, Cayer, U, Järlfors   +3 more
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Freeze-fracture techniques

1991
Abstract Freeze-fracture techniques were developed originally to prepare cellular ultrastructures in a frozen-hydrated and living state for electron microscopy (I). The replicas made from freeze-fractured biological specimens have provided a new three-dimensional ultrastructure of cells (2).
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Freeze-fracture morphology of nuclear pockets

Virchows Archiv B Cell Pathology Including Molecular Pathology, 1991
Nuclear pockets (NP) are found in numerous human tumours and in certain non-neoplastic cells. This study concerns the structure of NP in cells from two malignant rhabdoid tumours, one embryonal rhabdomyosarcoma, two centroblastic/centrocytic lymphomas, one centrocytic lymphoma and one Ki-1 lymphoma, as well as in normal neutrophils.
E, Kaiserling, H, Wolburg
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A technique for the freeze-fracture of tissue culture

Journal of Cell Science, 1978
ABSTRACT Cultures of rat cerebellum grown on glass coverslips coated first with collodion and then collagen are fixed in glutaraldehyde to which ruthenium red has been added. After fixation, the collodion comes off the glass coverslips and the 3 layers: collodion-collagen-culture are handled as one piece. Following immersion in glycerol,
L, Prescott, M W, Brightman
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Freeze-Fracture of Circulating Human Eosinophils

Acta Haematologica, 2009
Freeze-fracture has been carried out on peripheral blood from three patients with eosinophilia. Eosinophil granule membranes are characterized by intramembranous particles (IMP) with diameters of 80–150 Å. These particles of the granule membrane are consistently larger than the IMP of the plasma membrane which are approximately 80 Å in diameter.
S D, Douglas, M P, Ooka
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Freeze-Fracture Transmissionselektronenmikroskopie

2017
Der zweite Teil der vorliegenden Masterarbeit befasst sich mit der Untersuchung von drei Flussigkristallen mittels Freeze-Fracture Transmissionselektronenmikroskopie (FFTEM). Die untersuchten Mesogene sind in Tabelle 5.1 aufgelistet. Die Technik der Freeze-Fracture Transmissionselektronenmikroskopie wurde ursprunglich fur biologische Systeme entwickelt
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Preparing Hydra for Freeze-Fracture and Freeze-Etching

1983
The freeze-fracture technique is particularly advantageous for the study of cell surfaces and intercellular junctions. In general, it involves the rapid freezing of tissues, either with or without partial sublimation of ice (etching). By evaporating a film of pure carbon on the exposed surface, a replica of the cell surface is obtained which reveals ...
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