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Freezing and freeze-drying of microorganisms
Cryobiology, 1964Some papers reporting the effects of intense cold on microorganisms had already appeared in the 19th century and since then there have been a great many investigations of the freezing of microorganisms.3* 53 ‘I, 12 These experiments demonstrate that a wide variety of microorganisms can survive cooling to and storage at very low temperatures.
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Freeze-Substitution and Freeze-Drying
1987Freeze-substitution (FS) and freeze-drying (FD) are dehydration techniques by which the water is gently removed from a frozen specimen. Both techniques can serve as a link between cryofixation and conventional thin sectioning at room temperature (Fig. 1).
Steinbrecht, R., Müller, M.
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2016
Freezing of biological materials is commonly carried out to delay biochemical and chemical changes resulting in loss of activity. However, the physicochemical background of freezing is not well understood. Freeze-drying is generally aimed at preserving biological materials and bioactive components for long periods of time without the need for frozen ...
Yrjö H. Roos +2 more
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Freezing of biological materials is commonly carried out to delay biochemical and chemical changes resulting in loss of activity. However, the physicochemical background of freezing is not well understood. Freeze-drying is generally aimed at preserving biological materials and bioactive components for long periods of time without the need for frozen ...
Yrjö H. Roos +2 more
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2012
Fil: Martino, Miriam Nora. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos.
Ramallo, Laura Ana +2 more
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Fil: Martino, Miriam Nora. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos.
Ramallo, Laura Ana +2 more
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Freezing and Freeze-Drying of Bull Spermatozoa
American Journal of Physiology-Legacy Content, 1957The possible dependance of survival upon the time spermatozoa are in contact with the protective substance glycerol, prior to exposure to low temperatures, was investigated. Pretreatment for 5 minutes provided at least as much protection as 18 hours.
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European Journal of Obstetrics & Gynecology and Reproductive Biology, 2004
Blastocyst are preimplantation embryos that have successfully passed the critical step of genomic activation and have so a high developmental potential, thus allowing to reduce the number of embryos transferred. Blastocyst culture and freezing have really started in IVF programs in the early nineties, when it was possible to obtain good blastocysts ...
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Blastocyst are preimplantation embryos that have successfully passed the critical step of genomic activation and have so a high developmental potential, thus allowing to reduce the number of embryos transferred. Blastocyst culture and freezing have really started in IVF programs in the early nineties, when it was possible to obtain good blastocysts ...
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Journal of Microscopy, 1982
SUMMARYFreeze‐substitution is a technique suitable for the preparation of unicellular and multicellular plant and animal specimens for conventional light microscopy, TEM and SEM. It is also widely used as a means of preparing animal and plant tissues for the localization of water soluble substances by analytical electron microscopy, autoradiography or ...
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SUMMARYFreeze‐substitution is a technique suitable for the preparation of unicellular and multicellular plant and animal specimens for conventional light microscopy, TEM and SEM. It is also widely used as a means of preparing animal and plant tissues for the localization of water soluble substances by analytical electron microscopy, autoradiography or ...
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Archives of Neurology, 1991
The Journal of the American Medical Association and its family of specialty journals are addressing the issue of access to medical care. Matthew Menken presents a thoughtful look at this problem in a special article in this issue. 1 One of the major priorities in most industrialized nations is the provision of universal access to high-quality medical ...
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The Journal of the American Medical Association and its family of specialty journals are addressing the issue of access to medical care. Matthew Menken presents a thoughtful look at this problem in a special article in this issue. 1 One of the major priorities in most industrialized nations is the provision of universal access to high-quality medical ...
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Enzyme storage—to freeze or not to freeze?
Biochemical Society Transactions, 1989J P, Hill, F M, Dickinson
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