Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Effects of Freezing on Soil Temperature, Freezing Front Propagation and Moisture Redistribution in Peat: Laboratory Investigations [PDF]
, 2012 There are not many studies that report water movement in freezing peat. Soil column studies under controlled laboratory settings can help isolate and understand the effects of different factors controlling freezing of the active layer in organic covered ...
Hayashi, Masaki +3 more
core +1 more source
, 2004 Zero temperature dynamics of two dimensional triangulations of a torus with curvature energy is described. Numerical simulations strongly suggest that the model get frozen in metastable states, made of topological defects on flat surfaces, that group ...
Kownacki, J-P.
core +4 more sources
Updated observational constraints on quintessence dark energy models [PDF]
, 2018 The recent GW170817 measurement favors the simplest dark energy models, such as a single scalar field. Quintessence models can be classified in two classes, freezing and thawing, depending on whether the equation of state decreases towards $-1$ or ...
76283 +7 more
core +2 more sources
Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source
Color changes of blackberry as affected by freezing rate [PDF]
Acta Periodica Technologica, 2008 Fast frozen blackberry partially change its surface color from characteristic black to copper-redish color. This change decreases the market value, especially when the product is intended for export.
Ranđelović Dobrila G. +2 more
doaj +1 more source
Experimental study of the role of physicochemical surface processing on the IN ability of mineral dust particles [PDF]
, 2011 During the measurement campaign FROST 2 (FReezing Of duST 2), the Leipzig Aerosol Cloud Interaction Simulator (LACIS) was used to investigate the influence of various surface modifications on the ice nucleating ability of Arizona Test Dust (ATD ...
Buchholz, Angela +18 more
core +4 more sources
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics
Gut Microbes, 2022 Biobanking of live microbiota is becoming indispensable for mechanistic and clinical investigations of drug–microbiome interactions and fecal microbiota transplantation.
Xu Zhang +6 more
doaj +1 more source
Colligative properties of solutions: I. Fixed concentrations [PDF]
, 2005 Using the formalism of rigorous statistical mechanics, we study the phenomena of phase separation and freezing-point depression upon freezing of solutions.
A. Dembo +30 more
core +3 more sources