Förster Resonance Energy Transfer (FRET) Correlates of Altered Subunit Stoichiometry in Cys-Loop Receptors, Exemplified by Nicotinic α4β2 [PDF]
, 2012 We provide a theory for employing Förster resonance energy transfer (FRET) measurements to determine altered heteropentameric ion channel stoichiometries in intracellular compartments of living cells.
Akrap +51 more
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What We Fret About When We Fret About Bootstrapping [PDF]
, 2012 Den som färdas i staden har rätt att få svar på frågan: När kommer jag fram? Inställda turer, förseningar, trafikstockningar och brist på lediga parkeringsplatser försinkar oftast utan förvarning. Det är hög tid att förbättra tjänstens kvalité.
Blocher, Joseph
core +2 more sources
Inhibition of SARS-CoV 3CL protease by flavonoids
Journal of Enzyme Inhibition and Medicinal Chemistry, 2020 There were severe panics caused by Severe Acute Respiratory Syndrome (SARS) and Middle-East Respiratory Syndrome-Coronavirus. Therefore, researches targeting these viruses have been required.
Seri Jo +3 more
doaj +1 more source
Development of a Fluorescent Protein Based FRET Biosensor for Determination of Protease Activity
Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2021 Proteases are closely associated with many pathological conditions. Efficient detection of protease activity may be useful for diagnosis, prognosis, and the development of new therapeutic biomolecules.
İsa Gökçe +3 more
doaj +1 more source
Simultaneous quantitative live cell imaging of multiple FRET-based biosensors. [PDF]
PLoS ONE, 2013 We have developed a novel method for multi-color spectral FRET analysis which is used to study a system of three independent FRET-based molecular sensors composed of the combinations of only three fluorescent proteins.
Andrew Woehler
doaj +1 more source
Nanosecond Dynamics of Single-Molecule Fluorescence Resonance Energy Transfer
, 2004 Motivated by recent experiments on photon statistics from individual dye pairs planted on biomolecules and coupled by fluorescence resonance energy transfer (FRET), we show here that the FRET dynamics can be modelled by Gaussian random processes with ...
Agarwal, G. S. +4 more
core +1 more source
FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound
Sensors, 2020 Fluorescence resonance energy transfer (FRET)-based biosensors have advanced live cell imaging by dynamically visualizing molecular events with high temporal resolution.
Sangpil Yoon +3 more
doaj +1 more source
Fluorescence energy transfer enhancement in aluminum nanoapertures
, 2015 Zero-mode waveguides (ZMWs) are confining light into attoliter volumes, enabling single molecule fluorescence experiments at physiological micromolar concentrations. Among the fluorescence spectroscopy techniques that can be enhanced by ZMWs, F\"{o}rster
de Torres, Juan +4 more
core +3 more sources
ER/K linked GPCR-G protein fusions systematically modulate second messenger response in cells. [PDF]
, 2017 FRET and BRET approaches are well established for detecting ligand induced GPCR-G protein interactions in cells. Currently, FRET/BRET assays rely on co-expression of GPCR and G protein, and hence depend on the stoichiometry and expression levels of the ...
Dysthe, Matthew +4 more
core +1 more source
Anomalous surplus energy transfer observed with multiple FRET acceptors. [PDF]
PLoS ONE, 2009 BACKGROUND:Förster resonance energy transfer (FRET) is a mechanism where energy is transferred from an excited donor fluorophore to adjacent chromophores via non-radiative dipole-dipole interactions.
Srinagesh V Koushik +2 more
doaj +1 more source