Results 21 to 30 of about 229,738 (330)

Quantum Dots (QD-Dye Conjugate) are Ideal Candidates for FRET Investigations Using TIRMicroscopy [PDF]

open access: yesEngineering and Technology Journal, 2014
Total Internal Reflection Microscopy has been employed to investigate Förster resonance energy transfer FRET for QD-dye conjugate system. QDs based donor systems are ideal for FRET for many reasons, one of them is that their broad excitation cross ...
Asma H. Mohammed
doaj   +1 more source

Detection of Aflatoxins B1 in Maize Grains Using Fluorescence Resonance Energy Transfer

open access: yesApplied Sciences, 2020
Aflatoxins are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. These fungal species are the most dangerous and common toxin group causing food contamination. Aflatoxin has high toxicity and can cause cancer to humans and animals.
Thanh Binh Nguyen   +5 more
doaj   +1 more source

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET [PDF]

open access: yes, 2010
Flp-In T-REx 293 cells expressing a wild type human M muscarinic acetylcholine receptor construct constitutively and able to express a Receptor Activated Solely by Synthetic Ligand (RASSL) form of this receptor on demand maintained response to the ...
Alvarez-Curto, Elisa   +3 more
core   +2 more sources

A FRET Based Two-Photon Fluorescent Probe for Visualizing Mitochondrial Thiols of Living Cells and Tissues

open access: yesSensors, 2020
Glutathione (GSH) is the main component of the mitochondrial thiol pool and plays key roles in the biological processes. Many evidences have suggested that cysteine and homocysteine also exist in mitochondria and are interrelated with GSH in biological ...
Zhengkun Liu   +4 more
doaj   +1 more source

Inhibition of SARS-CoV 3CL protease by flavonoids

open access: yesJournal of Enzyme Inhibition and Medicinal Chemistry, 2020
There were severe panics caused by Severe Acute Respiratory Syndrome (SARS) and Middle-East Respiratory Syndrome-Coronavirus. Therefore, researches targeting these viruses have been required.
Seri Jo   +3 more
doaj   +1 more source

Light-harvesting nanoparticle probes for FRET-based detection of oligonucleotides with single-molecule sensitivity.

open access: yesAngewandte Chemie, 2020
Controlling emission of bright luminescent nanoparticles by a single molecular recognition event remains an ultimate challenge in the design of ultrasensitive probes for biomolecules. It would require an efficient Förster resonance energy transfer (FRET)
N. Melnychuk   +4 more
semanticscholar   +1 more source

A rationally enhanced red fluorescent protein expands the utility of FRET biosensors

open access: yesNature Communications, 2020
Genetically encoded Förster Resonance Energy Transfer (FRET)-based biosensors are powerful tools to illuminate spatiotemporal regulation of cell signaling in living cells, but the utility of the red spectrum for biosensing was limited due to a lack of ...
G. Mo   +4 more
semanticscholar   +1 more source

Investigation of a Direct Interaction between miR4749 and the Tumor Suppressor p53 by Fluorescence, FRET and Molecular Modeling

open access: yesBiomolecules, 2020
The interactions between the DNA binding domain (DBD) of the tumor suppressor p53 and miR4749, characterized by a high sequence similarity with the DNA Response Element (RE) of p53, was investigated by fluorescence spectroscopy combined with ...
Anna Rita Bizzarri   +1 more
doaj   +1 more source

In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET

open access: yesBiophysical Reports, 2023
Förster resonance energy transfer (FRET) microscopy is used in numerous biophysical and biomedical applications to monitor inter- and intramolecular interactions and conformational changes in the 2–10 nm range. FRET is currently being extended to in vivo
Jason T. Smith   +5 more
doaj   +1 more source

Protein-Protein Affinity Determination by Quantitative FRET Quenching. [PDF]

open access: yes, 2019
The molecular dissociation constant, Kd, is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions.
Chen, Younan   +7 more
core   +2 more sources

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