Results 21 to 30 of about 3,549 (237)

Transforming a Fructan:Fructan 6G-Fructosyltransferase from Perennial Ryegrass into a Sucrose:Sucrose 1-Fructosyltransferase   [PDF]

Plant Physiology, 2008
AbstractFructosyltransferases (FTs) synthesize fructans, fructose polymers accumulating in economically important cool-season grasses and cereals. FTs might be crucial for plant survival under stress conditions in species in which fructans represent the major form of reserve carbohydrate, such as perennial ryegrass (Lolium perenne). Two FT types can be
Lasseur, Bertrand, Schroeven, Lindsey, Lammens, Willem, Le Roy, Katrien, Spangenberg, German, Manduzio, Helene, Vergauwen, Rudolf, Lothier, Jeremy, Prud'homme, Marie-Pascale, Van den Ende, Wim   +9 more
openaire   +3 more sources

Simultaneous Knockout of Tk1-SST and Tk1-FFT via CRISPR/Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok-saghyz. [PDF]

Plant Biotechnol J
ABSTRACT Taraxacum kok‐saghyz (TKS) synthesises natural rubber (NR) and inulin using sucrose as a carbon source. However, molecular mechanisms regulating inulin and NR accumulation remain largely unclear. Here, we report the generation of double‐gene homozygous mutants, 1‐sst1‐fft, by simultaneously knocking out two key genes responsible for inulin ...
Qin B, Hou X, Fan S, Li C, Wang X, Chen Q, Yang Y, Lin P, Lu LR, Fu C, Yang N, Gao Q, Zheng S, Liu S.   +13 more
europepmc   +2 more sources

Identification and structural basis of an enzyme that degrades oligosaccharides in caramel

Biophysics and Physicobiology, 2023
Cooking with fire produces foods containing carbohydrates that are not naturally occurring, such as α-d-fructofuranoside found in caramel. Each of the hundreds of compounds produced by caramelization reactions is considered to possess its own ...
Toma Kashima, Akihiro Ishiwata, Kiyotaka Fujita, Shinya Fushinobu   +3 more
doaj   +1 more source

Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase

Catalysts, 2023
The catalytic properties of Seqenzym® FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5.
Klaudia Karkeszová, M. Polakovič
semanticscholar   +1 more source

Presence of Inulin-Type Fructo-Oligosaccharides and Shift from Raffinose Family Oligosaccharide to Fructan Metabolism in Leaves of Boxtree (Buxus sempervirens) [PDF]

, 2016
from raffinose family oligosaccharide to fructan metabolism in leaves of boxtree (Buxus sempervirens) Wim Van den Ende1,* Marlies Coopman1, Rudy Vergauwen1, André Van Laere11 KU Leuven, Laboratory of Molecular Plant Biology, Institute of Botany and ...
André Van Laere, Marlies Coopman, Rudy Vergauwen, Wim Van den Ende   +3 more
core   +4 more sources

The differential expression of the two key genes involved in fructan biosynthetic pathway in artichoke vs. wild cardoon improves inulin-type fructans

Biologia Plantarum, 2023
The artichoke (Cynara cardunculus subsp. scolymus) is an intriguing source of indigestible sugar polymers such as inulin-type fructans. Artichoke represents an important component of a traditional Mediterranean diet and its edible parts are a good source
M. FERRARI, C. DE SIO, A. MUTO, S. PAGLIARI, I. BRUNI, L. BRUNO, M. LABRA, R. COZZA   +7 more
doaj   +1 more source

The large subunit determines catalytic specificity of barley sucrose:fructan 6‐fructosyltransferase and fescue sucrose:sucrose 1‐fructosyltransferase [PDF]

FEBS Letters, 2004
Plant fructosyltransferases are highly homologous in primary sequence and typically consist of two subunits but catalyze widely different reactions. Using functional expression in the yeast Pichia pastoris, we show that the substrate specificity of festuca sucrose:sucrose 1‐β‐d‐fructosyltransferase (1‐SST) and barley sucrose:fructan 6‐β‐d ...
Altenbach, Denise, Nüesch, Eveline, Meyer, Alain D., Boller, Thomas, Wiemken, Andres   +4 more
openaire   +2 more sources

Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana. [PDF]

PLoS ONE, 2012
Fructans are the main storage polysaccharides found in Agave species. The synthesis of these complex carbohydrates relies on the activities of specific fructosyltransferase enzymes closely related to the hydrolytic invertases. Analysis of Agave tequilana
Celso Cortés-Romero, Aída Martínez-Hernández, Erika Mellado-Mojica, Mercedes G López, June Simpson   +4 more
doaj   +1 more source

Microbial fructosyltransferases and the role of fructans [PDF]

Journal of Applied Microbiology, 2009
Microbial fructosyltransferases are polymerases that are involved in microbial fructan (levan, inulin and fructo-oligosaccharide) biosynthesis. Structurally, microbial fructosyltransferase proteins share the catalytic domain of glycoside hydrolases 68 family and are grouped in seven phylogenetically related clusters. Fructosyltransferase-encoding genes
M L, Velázquez-Hernández, V M, Baizabal-Aguirre, A, Bravo-Patiño, M, Cajero-Juárez, M P, Chávez-Moctezuma, J J, Valdez-Alarcón   +5 more
openaire   +2 more sources

Stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (Triticum aestivum L.) genotypes with contrasting tolerance to water stress. [PDF]

PLoS ONE, 2017
The genetic and physiological mechanisms underlying the relationship between water-soluble carbohydrates (WSC) and water stress tolerance are scarcely known.
Alejandra Yáñez, Gerardo Tapia, Fernando Guerra, Alejandro Del Pozo   +3 more
doaj   +1 more source