Results 151 to 160 of about 339,629 (251)

Targeting Protein-Protein Interactions (PPIs) to Drive Functional Annotation: An Integrative Methodology to Study Senescence-Associated PPIs Using TMPRSS11a as Model Interactor. [PDF]

Biomolecules
Rosales-Rojas R, Fernández C, González-Avendaño M, Vergara-Jaque A, Cáceres M.   +4 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Genome-wide adaptive selection and functional annotation of regulatory variation in the Yangxin pig. [PDF]

BMC Genomics
Liu S, Chen Q, Zhou H, Yang C, Shi L, Chen H.   +5 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Functional annotation of novel heat stress-responsive genes in rice utilizing public transcriptomes and structurome. [PDF]

Bioinform Adv
Yonezawa S, Bono H.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Step-by-Step Computational Protocol for Functional Annotation and Structural Modelling of Insect Chemosensory Proteins. [PDF]

Bio Protoc
Kalepu R, Hamid AAA, Hassan M, Mohd-Assaad N, Muhammad NAN.   +4 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Functional Annotation Analytics of Rhodopseudomonas palustris Genomes

Bioinformatics and Biology Insights, 2011
Shaneka S. Simmons, Raphael D. Isokpehi, Shyretha D. Brown, Donee L. McAllister, Charnia C. Hall, Wanaki M. McDuffy, Tamara L. Medley, Udensi K. Udensi, Rajendram V. Rajnarayanan, Wellington K. Ayensu, Hari H.P. Cohly   +10 more
doaj  

Transcriptomic and functional annotation datasets for GV1001 peptide treatment in an experimental autoimmune encephalomyelitis mouse model. [PDF]

Data Brief
Kim JH, Zheng LT, Kim S, Suk K.
europepmc   +1 more source