Results 51 to 60 of about 845,849 (268)
Cassette hybridization for vector assembly application in antibody chain shuffling
BioTechniques, 2018 Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials.
Jing Yi Lai +3 more
doaj +1 more source
The human gut microbiome across the life course
FEBS Letters, EarlyView.Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero +4 more
wiley +1 more source
A New Nonsmooth Bundle-Type Approach for a Class of Functional Equations in Hilbert Spaces
Journal of Function Spaces, 2017 A new bundle-type approach for solving a class of functional equations is presented by combining bundle idea for nonsmooth optimization with common iterative process for functional equations.
Jie Shen +3 more
doaj +1 more source
Eigenvalue Problem for the Second Order Differential Equation with Nonlocal
Nonlinear Analysis, 2006 The paper deals with numerical methods for eigenvalue problem for the second order ordinary differential operator with variable coefficient subject to nonlocal integral condition.
B. Bandyrskii +3 more
doaj +1 more source
An Iterative Method for Finding Stationary Values of a Function of Several Variables [PDF]
The Computer Journal, 1962 Eighteen months ago Rosenbrock (1960) published a paper in this journal on finding the greatest or least value of a function of several variables. A number of methods were listed and they all have first-order convergence. Six months ago Martin and Tee (1961) published a paper in which they mentioned gradient methods which have second-order convergence ...
openaire +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
The exact soliton wave solutions of the nonlinear evolution equation with variable coefficients
Examples and CounterexamplesIn this article, we investigated new traveling wave solutions of the modified Zakharov–Kuznetsov equation with an additional time-dependent variable coefficient using the functional variable method.
Fakhriddin Abdikarimov
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source