Comparison of methods for isolating fungal DNA [PDF]
Czech Journal of Food Sciences, 2011 In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj +4 more sources
An overview of using fungal DNA for the diagnosis of invasive mycoses
Expert Review of Molecular Diagnostics, 2022 Introduction Fungal PCR has undergone considerable standardization and, together with the availability of commercial assays, external quality assessment schemes, and extensive performance validation data, is ready for widespread use for the screening and
P. White +12 more
semanticscholar +6 more sources
Rhinitis, Ocular, Throat and Dermal Symptoms, Headache and Tiredness among Students in Schools from Johor Bahru, Malaysia: Associations with Fungal DNA and Mycotoxins in Classroom Dust. [PDF]
PLoS ONE, 2016 There are few studies on rhinitis and sick building syndrome (SBS) among students in tropical countries. We studied associations between levels of five fungal DNA sequences, two mycotoxins (sterigmatocystin and verrucarol) and cat allergen (Fel d 1 ...
Dan Norbäck +6 more
doaj +2 more sources
Rapid extraction of fungal DNA for PCR amplification.
Nucleic Acids Research, 1992 Polymerase chain reaction (PCR) is a technique widely used in fungal research. One of its advantages is the ability to amplify very small amounts of DNA, in the picograms range, even in the presence of diverse contaminants.
J. L. Cénis
semanticscholar +6 more sources
Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification [PDF]
Open Life SciencesGenomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani +5 more
doaj +2 more sources
GC Content of the Rumen Fungal DNA [PDF]
CrossRef Listing Of Deleted DOIs, 1995 V. Kostyukovsky +5 more
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Influence of DNA extraction kits on freshwater fungal DNA metabarcoding. [PDF]
PeerJ, 2022 AbstractBackgroundEnvironmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Matsuoka S, Sugiyama Y, Nagano M, Doi H.
europepmc +6 more sources
Use of magnetic beads to extract fungal DNA [PDF]
Mycoses, 2005 SummaryAuthors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T.
Elisabetta Faggi +2 more
openalex +6 more sources
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA. [PDF]
J Vis Exp, 2018 Lemons AR, Lindsley WG, Green BJ.
europepmc +3 more sources