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Candida glabrata: A Lot More Than Meets the Eye
Microorganisms, 2019 Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar +3 more
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Rapid Mini-Preparation of Fungal DNA for PCR [PDF]
Journal of Clinical Microbiology, 2000 Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
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General Fungal DNA Extraction v2
, 2023 This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias +2 more
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Mycelial DNA persistence in a forest soil
Environmental DNA, 2021 Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
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Evolutionary Relationship and the Sequence Similarities among Different Fungal Species Infecting Birds Captured from Different Areas in Denmark [PDF]
Archives of Razi Institute, 2022 Fungal diseases are the common cause of death in wild animals and birds of prey. This study was designed to investigate the development of fungal infections among wild birds in Denmark.
Z Shaker Al-Rubaiee +2 more
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Using DNA Barcoding in Fungal Taxonomy
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020 Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
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Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]
Journal of Clinical Microbiology, 1999 ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
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mBio, 2021 Although relatively few in number, studies of DNA methylation have shown that fungal DNA methylation is implicated in development, genome integrity, and genome defense.
Yo-Han Ko +3 more
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Fungal DNA Extraction from Fungal Plate v1
, 2020 This protocol describes how to do fungal DNA extraction from fungal plate. This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F ...
You Li, Demian F Gomez
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Beringian Paleoecology Inferred from Permafrost-Preserved Fungal DNA [PDF]
Applied and Environmental Microbiology, 2005 ABSTRACT The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used.
Lydolph, Magnus C +7 more
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