Results 21 to 30 of about 2,640,436 (349)

Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]

open access: bronzeJournal of Clinical Microbiology, 1999
ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
Dagmar Rimek   +3 more
openalex   +4 more sources

Epigenetic Regulation of Antifungal Drug Resistance

open access: yesJournal of Fungi, 2022
In medical mycology, epigenetic mechanisms are emerging as key regulators of multiple aspects of fungal biology ranging from development, phenotypic and morphological plasticity to antifungal drug resistance.
Sandip Patra   +3 more
doaj   +1 more source

An Assay to Determine NAD(P)H: Quinone Oxidoreductase Activity in Cell Extracts from Candida glabrata

open access: yesBio-Protocol, 2021
Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans.
Anamika Battu   +2 more
doaj   +1 more source

Unraveling the ancient fungal DNA from the Iceman gut. [PDF]

open access: yesBMC Genomics
AbstractHere, we explore the possible ancient fungal species in the gut of Ötzi, the Iceman, a naturally mummified human found in the Tyrolean Alps (border between Italy and Austria). While ancient DNA (aDNA) has been extensively used to study human, animal, and plant evolution, this research focuses on ancient microbial diversity, specifically fungi ...
Oskolkov N   +8 more
europepmc   +4 more sources

SUMOylation in Human Pathogenic Fungi: Role in Physiology and Virulence

open access: yesJournal of Fungi, 2020
The small ubiquitin-related modifier (SUMO) protein is an important component of the post-translational protein modification systems in eukaryotic cells.
Mahima Sagar Sahu   +3 more
doaj   +1 more source

The use of flow cytometry for fungal nuclear DNA quantification [PDF]

open access: yesCytometry Part A, 2021
AbstractGenome size information is sparse across fungi, with information being available for less than 2000 species. So far, most records have been obtained using static, microscope‐based cytometry methods or derived from genome sequencing projects. Flow cytometry is now considered the state‐of‐the‐art method for obtaining genome size measurements, and
Pedro Talhinhas   +2 more
openaire   +3 more sources

The Quest for a General and Reliable Fungal DNA Barcode [PDF]

open access: yesThe Open Applied Informatics Journal, 2011
DNA sequences are key elements for both identification and classification of living organisms. Mainly for historical reasons, a limited number of genes are currently used for this purpose. From a mathematical point of view, any DNA segment, at any location, even outside of coding regions and even if they do not align, could be used as long as PCR ...
V. Robert   +7 more
openaire   +5 more sources

Fungal CTAB DNA Extraction v1 [PDF]

open access: yes, 2020
This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House   +3 more
openaire   +2 more sources

Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations

open access: yesBio-Protocol, 2023
The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant
Hosung Jeon, Hokyoung Son, Kyunghun Min
doaj   +1 more source

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