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Fusion proteins in biotechnology
Current Opinion in Biotechnology, 1992Gene fusion techniques allow the production of recombinant proteins featuring the combined characteristics of the parental products. Originally, these techniques were used to probe transcriptional and translational activity, to translocate proteins across cell membranes, and to facilitate the recovery of proteins.
M, Uhlén +4 more
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Fusion-protein-assisted protein crystallization [PDF]
Acta Crystallographica Section F, Structural Biology Communications, 2015 Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the `heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation.
Boštjan Kobe +2 more
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An optimized antigen–protein fusion
Nature Biomedical Engineering, 2020The fusion of an immunogenic peptide and the protein transthyretin protects the peptide antigen from proteolytic degradation, optimizes its uptake in local draining lymphatics and reduces its presentation in uninflamed distal lymphoid organs, as shown in mice.
Pedro Romero +2 more
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DEPLOYMENT OF MEMBRANE FUSION PROTEIN DOMAINS DURING FUSION
Cell Biology International, 2000AbstractIt is clear that both viral and intracellular membrane fusion proteins contain a minimal set of domains which must be deployed at the appropriate time during the fusion process. An account of these domains and their functions is given here for the four best‐described fusion systems: influenza HA, sendai virus F1, HIV gp120/41 and the neuronal ...
J, Bentz, A, Mittal
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Preparation of GST Fusion Proteins
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol describes the preparation of glutathione-S-transferase (GST) fusion proteins, which have had a wide range of applications since their introduction as tools for synthesis of recombinant proteins in bacteria. GST was originally selected as a fusion moiety because of several desirable properties.
Margret B, Einarson +2 more
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Construction of Immunoglobulin Fusion Proteins
Current Protocols in Immunology, 2002AbstractRecombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a ...
Diane, Hollenbaugh, Alejandro, Aruffo
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Protein-Mediated Membrane Fusion
Annual Review of Biophysics and Biophysical Chemistry, 1989PERSPECTIVES AND OVERVIEW 187 GEN ERAL CON CEPTS 188 Fusion Versus Fission 188 Endoplasmic and Exoplasmic Fusion Events 189 Tightness of Fusion 190 PHYSICAL FORCES BETWEEN MEMBRAN ES ........ 190 FUSION ASSAyS 191 LlPOSOMES AS TARGET MEMBRANES 192 EXOPLASMIC FUSION 193 Influenza Virus Fusion 193 Acid-Induced Conformational Change in ...
T, Stegmann, R W, Doms, A, Helenius
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Bone Morphogenetic Protein and Fusion
Journal of Neurosurgery: Spine, 2007In their article (Villavicencio AT, Burneikiene S, Nelson EL, et al: Safety of transforaminal lumbar interbody fusion and intervertebral recombinant human bone morphogenetic protein– 2. J Neurosurg Spine 3: 436– 443, December, 2005), Villavicencio and colleagues referred to the work by Haid et al., claiming that the fusion rates during ...
Pećina, Marko +2 more
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Diphtheria Toxin Fusion Proteins
1998Two different approaches have been undertaken to develop targeted biomolecules for therapeutics. The first was the construction of immunotoxins consisting of monoclonal antibodies chemically linked through a disulfide bond to a plant or bacterial toxin or radionuclide.
F M, Foss +4 more
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Antibody–cytokine fusion proteins
Archives of Biochemistry and Biophysics, 2012Cytokines are key players in stimulating and regulating immune responses in physiological and pathophysiological processes. Various cytokines have been approved for therapy of cancer and other diseases and many more are under development. However, therapeutic efficacy is often hampered by severe side effects and poor pharmacokinetic properties.
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