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Preparation of Insoluble GST Fusion Proteins
Cold Spring Harbor Protocols, 2009INTRODUCTIONA wide variety of plasmid vectors are commercially available for the production of fusion proteins in bacterial cells. Most are also designed to incorporate a tag that allows affinity purification of the expressed fusion protein from bacterial cell extracts.
Ilaria, Rebay, Richard G, Fehon
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Quantitative lmmunoblotting with Fusion Proteins
2003The development of vaccines against HIV-1 is currently hindered by incomplete understanding of correlates of protective immunity (1-3). Experiments are necessary to measure immune responses in sufficiently fine detail that specific protective responses can be discerned from those that are irrelevant or harmful.
M M, Mitchell, L D, Loomis-Price
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Cytokine Fusion Protein Treatment
2002Mice suffering from melanoma can be cured by treatment with a recombinant antibody-lymphotoxin-alpha fusion protein. In order to characterize the involvement of T cells in this syngeneic tumor model, the T cell receptor repertoire was analyzed both quantitatively and qualitatively.
David, Schrama +4 more
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Design of fusion proteins for biocatalysis
The use of enzymes to convert substrates into valuable products has been an integral part of biocatalysis. However, some reactions are energy-demanding that requires the use of NAD(P)H to proceed. This NAD(P)H can be costly impeding the progress of enzyme usage at a bigger scale.Beyzanur, Celebi +3 more
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Fusion of classifiers for predicting protein–protein interactions
Neurocomputing, 2005Prediction of protein-protein interaction is a difficult and an important problem in biology. In this paper, we describe a very general method for predicting protein-protein interactions. The interaction mining approach is demonstrated by building a learning system based on experimentally validated protein-protein interactions in the human gastric ...
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Fusion Proteins in Retroviral Transformation
1985Publisher Summary Retroviral transforming proteins are coded for by oncogenes. The mechanism of the uptake of oncogenes by retroviruses often results in fusion with replicative genes. Fusion of oncogenes to retroviral replicative genes generates protein products that are also fused.
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The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein
Cell, 2017Juliette Fedry, Yanjie Liu, Jimin Pei
exaly