Results 21 to 30 of about 1,121,449 (385)

Lysis of Staphylococcal Cells by Modular Lysin Domains Linked via a Non-covalent Barnase-Barstar Interaction Bridge

Frontiers in Microbiology, 2019
Bacteriophage endolysins and bacterial exolysins are capable of enzymatic degradation of the cell wall peptidoglycan layer and thus show promise as a new class of antimicrobials. Both exolysins and endolysins often consist of different modules, which are
Linnea C. Hjelm, Johan Nilvebrant, Per-Åke Nygren, Anders S. Nilsson, Johan Seijsing   +4 more
doaj   +1 more source

Targeted delivery of autoantigen to dendritic cells prevents development of spontaneous uveitis

Frontiers in Immunology, 2023
Restoration of immunological tolerance to self antigens has been a major drive in understanding the mechanisms of, and developing new treatments for, autoimmune and autoinflammatory disease.
Izabela P. Klaska, Tian Yu, Tian Yu, Rosie Fordyce, Koju Kamoi, Koju Kamoi, Richard J. Cornall, Cristina Martin-Granados, Lucia Kuffova, Lucia Kuffova, John V. Forrester   +10 more
doaj   +1 more source

Protein-Protein Fusion Catalyzed by Sortase A

PLoS ONE, 2011
Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of ...
Ranganath Parthasarathy, David Levary, Eric T. Boder, Margaret E. Ackerman   +3 more
openaire   +5 more sources

ATP-independent Control of Vac8 Palmitoylation by a SNARE Subcomplex on Yeast Vacuoles [PDF]

, 2005
Yeast vacuole fusion requires palmitoylated Vac8. We previously showed that Vac8 acylation occurs early in the fusion reaction, is blocked by antibodies against Sec18 (yeast N-ethylmaleimide-sensitive fusion protein (NSF)), and is mediated by the R-SNARE
Cristodero, Marina, Dietrich, Lars E. P., LaGrassa, Tracy J., Meiringer, Christoph T. A., Rohde, Jan, Ungermann, Christian   +5 more
core   +1 more source

Enhanced Phycocyanobilin Production in Escherichia coli by Fusion-Expression of Apo-Proteins with Signal Peptides

Fermentation, 2023
Phycocyanobilin (PCB) is the bioactive chromophore attached to Phycocyanin (PC) that is of special interest for nutraceutical and therapeutic applications. However, the production of PCB from the heterologous host Escherichia coli is still very low.
Xiaolin Liu, Jing Yu, Qian Che, Tianjiao Zhu, Dehai Li, Guojian Zhang   +5 more
doaj   +1 more source

Construction of a Collagen-like Protein Based on Elastin-like Polypeptide Fusion and Evaluation of Its Performance in Promoting Wound Healing

Molecules, 2023
In the healing of wounds, human-like collagen (hCol) is essential. However, collagen-based composite dressings have poor stability in vivo, which severely limits their current therapeutic potential.
Yingli Chen, Yuanyuan Wu, Fengmin Xiong, Wei Yu, Tingting Wang, Jingjing Xiong, Luping Zhou, Fei Hu, Xianlong Ye, Xinmiao Liang   +9 more
doaj   +1 more source

Fusion of isolated myoblast plasma membranes [PDF]

, 1978
Fusion of plasma membranes isolated from myoblasts grown in culture has been investigated. 1. 1. Membrane fusion was specifically dependent on Ca2+ at physiological concentrations. However, at higher concentrations of cations, fusion could be triggered
Ahkong, Batzri, Berl, Berlin, Burwen, Croce, Dahl, Dahl, Dahl, De Camilli, Douglas, Dreifuss, Fairbanks, Gratzl, Gratzl, Gratzl, Hauser, Holtzer, Kalderon, Kantor, Kent, Lawson, Lee, Maeda, Martin, Okada, Okada, Onishi, Orci, Pagano, Papahadjopoulos, Papahadjopoulos, Papahadjopoulos, Papahadjopoulos, Pauli, Peretz, Poole, Poste, Prives, Rash, Reed, Reeves, Sanger, Satir, Schimmel, Schlessinger, Schudt, Schudt, Schudt, Schudt, Shainberg, Shainberg, Van den Bosch, Van den Bosch, Van den Bosch, Vos, Weidekamm, Weidekamm, Yanofsky, Zakai   +59 more
core   +1 more source

Structural Insights into a Bifunctional Peptide Methionine Sulfoxide Reductase MsrA/B Fusion Protein from Helicobacter pylori

Antioxidants, 2021
Methionine sulfoxide reductase (Msr) is a family of enzymes that reduces oxidized methionine and plays an important role in the survival of bacteria under oxidative stress conditions.
Sulhee Kim, Kitaik Lee, Sun-Ha Park, Geun-Hee Kwak, Min Seok Kim, Hwa-Young Kim, Kwang Yeon Hwang   +6 more
doaj   +1 more source

Mycobacterium tuberculosis antigen 85B and ESAT-6 expressed as a recombinant fusion protein in Mycobacterium smegmatis elicits cell-mediated immune response in a murine vaccination model [PDF]

, 2013
This is the post-print version of the final paper published in Molecular Immunology. The published article is available from the link below. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting ...
Aagaard, Agger, Andersen, Andersen, Anthony G. Tsolaki, Bennekov, Blokpoel, Brian D. Robertson, Carroll, Crowe, Curcic, Delogu, Delogu, DeMaio, Dietrich, Dye, Dziadek, Ehrt, Fine, Garbe, Gupta, Hanekom, Ida Rosenkrands, Judit Nagy, Laemmli, Langermans, Maher, Masungi, Menozzi, Menozzi, Okkels, Olsen, O’Donnell, Parish, Pethe, Roche, Russell, Sambrook, Sergio Leiva, Temmerman, Triccas, Triccas, Tsolaki, Uday Kishore, van Dissel, van Dissel, Weinrich Olsen, Wiles, Williams   +48 more
core   +1 more source

GENOTYPING AND GENETIC EVOLUTION ANALYSIS OF NEWLY ISOLATED NEWCASTLE DISEASE VIRUS IN EGYPT

Slovenian Veterinary Research, 2021
In spite of enormous vaccination programs that were implemented in the Egyptian poultry farms, Newcastle Disease Virus (NDV) remains one of the major concerns to the poultry industry.
Yahia Madbouly, Mohammed Shakal, Eman Aly, Ashraf Hussein, Mohammed Abdelsabour, Amal Eid   +5 more
doaj   +1 more source